Minakawa M, Sugimoto T, Aizawa S, Tomooka Y
Department of Biological Science and Technology, Science University of Tokyo, 2641 Yamazaki Noda, Chiba 278-8510, Japan.
Brain Res. 1998 Nov 30;813(1):172-6. doi: 10.1016/s0006-8993(98)00979-2.
p53-Deficient mice are prone to develop spontaneous tumors [L.A. Donehower, M. Harvey, B.L. Slagle, M.J. McArthur, C.A. Montgomery Jr. , J.S. Butel, A. Bradley, Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumors, Nature 356 (1992) 215-221], but brain neoplasms are rare and difficult to culture in vitro. Here we describe cloning and long-term culture of heterogeneous neural cell lines from a multifocal cerebellar neoplasm that arose in an adult p53-/- mutant mouse. These lines may be useful for studies of neoplastic transformation and cell lineage in cerebellar development.
p53基因缺陷型小鼠易于发生自发性肿瘤[L.A. 多内霍弗、M. 哈维、B.L. 斯拉格尔、M.J. 麦克阿瑟、C.A. 蒙哥马利二世、J.S. 布特尔、A. 布拉德利，p53基因缺陷型小鼠发育正常但易患自发性肿瘤，《自然》356卷（1992年）第215 - 221页]，但脑肿瘤罕见且难以在体外培养。在此，我们描述了从一只成年p53基因敲除突变小鼠发生的多灶性小脑肿瘤中克隆并长期培养异质性神经细胞系的过程。这些细胞系可能有助于研究小脑发育中的肿瘤转化和细胞谱系。
