Hoffmann T, Favre G, Gratwohl A, Passweg J, Mathys S, Borel J, Speck B, Tichelli A
Departement Zentrallabor, Kantonsspital, Basel.
Schweiz Med Wochenschr. 1998 Oct 17;128(42):1611-3.
Bone marrow samples used for flow cytometric analysis of plasma cells frequently provide a low plasma cell content. Regularly, samples used for flow cytometry are provided by second step aspiration while the first aspirate is used for cytologic examination. We investigated whether the use of secondary aspirates leads to a systematic underestimation of the bone marrow plasma cell content as a consequence of an increased blood contaminant. To test the hypothesis, plasma cell (CD38bright) percentages were established by flow cytometry in 13 pairs of primary/secondary aspirates. In all cases we found lower plasma cell contents in secondary as compared to primary aspirates (p = 0.0015). Median plasma cell counts in secondary aspirates were 57% lower compared to primary aspirates. We conclude that the use of secondary aspirates leads to systematic underestimation of the bone marrow plasma cell content.
用于浆细胞流式细胞术分析的骨髓样本通常浆细胞含量较低。通常，用于流式细胞术的样本由第二次抽吸提供，而第一次抽吸物用于细胞学检查。我们研究了由于血液污染物增加，使用二次抽吸物是否会导致骨髓浆细胞含量被系统性低估。为了验证这一假设，通过流式细胞术测定了13对初次/二次抽吸物中的浆细胞（CD38bright）百分比。在所有病例中，我们发现二次抽吸物中的浆细胞含量低于初次抽吸物（p = 0.0015）。二次抽吸物中的浆细胞计数中位数比初次抽吸物低57%。我们得出结论，使用二次抽吸物会导致骨髓浆细胞含量被系统性低估。
