Aktuğ T, Uçan B, Olguner M, Akgür F M, Ozer E
Department of Pediatric Surgery and Pathology, Dokuz Eylül University, Medical Faculty, Izmir, Turkey.
Eur J Pediatr Surg. 1998 Oct;8(5):308-11. doi: 10.1055/s-2008-1071220.
The prevention of intestinal damage in gastroschisis was reported after amnio-allantoic fluid (AAF) exchange using serum saline. However it is not clear why AAF exchange prevents intestinal damage. Are some harmful chemicals withdrawn from AAF by exchange or sodium content of AAF is merely increased? A study was planned to find the end results of AAF exchange performed by using two different solutions in the chicken embryo gastroschisis model. After creation of gastroschisis, three groups were studied. In the first group AAF exchange was not performed. In the second group AAF exchange was performed using 0.075% NaCl solution (physiologic saline for chicken). In the third group AAF exchange was performed using 0.054% NaCl plus 1% dextrose solution. Exchange with two different solutions prevented cellular infiltration and fibrous peel formation in intestines which are observed in the non-treatment group. In the third group, intestinal edema was an additional pathologic finding. This study shows that in gastroschisis, the intestines outside the abdominal cavity are very sensitive to the quality of the fluid in which they are bathed. The intestinal damage resembling that encountered in human gastroschisis can be prevented by removing the harmful chemicals from AAF by exchange.
据报道，使用血清盐水进行羊膜-尿囊液（AAF）置换后可预防腹裂患儿的肠道损伤。然而，目前尚不清楚为何AAF置换可预防肠道损伤。是通过置换从AAF中去除了某些有害化学物质，还是仅仅提高了AAF的钠含量？本研究旨在探究在鸡胚腹裂模型中使用两种不同溶液进行AAF置换的最终结果。制造腹裂模型后，对三组进行研究。第一组不进行AAF置换。第二组使用0.075% NaCl溶液（鸡用生理盐水）进行AAF置换。第三组使用0.054% NaCl加1%葡萄糖溶液进行AAF置换。用两种不同溶液进行置换可预防未治疗组中观察到的肠道细胞浸润和纤维性包膜形成。在第三组中，肠道水肿是另一个病理表现。本研究表明，在腹裂中，腹腔外的肠道对其所处液体的质量非常敏感。通过置换从AAF中去除有害化学物质可预防类似于人类腹裂中出现的肠道损伤。
