Uchida N, Mitani H, Shima A
Department of Biological Sciences, University of Tokyo, Japan.
Photochem Photobiol. 1998 Nov;68(5):725-8.
Previously we reported that fluorescent light (FL) illumination prior to UV irradiation (FL preillumination) had multiple effects on the repair of cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts ([6-4] PD) in cultured goldfish cells (RBCF-1) at the exponentially growing phase. In this study, it is shown that even under the confluent condition of RBCF-1 cells, FL preillumination increased the disappearance of (6-4) PD in the dark. In addition, both at confluence and at the exponentially growing phase, the disappearance of (6-4) PD after PR treatment was increased by FL preillumination to RBCF-1 cells. Moreover, it was found that H2O2 pretreatment, followed by UV irradiation, of the exponentially growing cells also enhanced the disappearance of (6-4) PD in the dark and by photorepair treatment. The degree of enhancement by H2O2 pretreatment was almost the same as that by FL preillumination.
此前我们报道，在指数生长期的培养金鱼细胞（RBCF-1）中，紫外线照射前的荧光（FL）照明（FL预照明）对环丁烷嘧啶二聚体（CPD）和（6-4）光产物（[6-4]PD）的修复有多种影响。在本研究中，结果表明，即使在RBCF-1细胞汇合的条件下，FL预照明也会增加黑暗中（6-4）PD的消失。此外，在汇合期和指数生长期，FL预照明RBCF-1细胞后，PR处理后（6-4）PD的消失均增加。此外，还发现对指数生长期细胞进行H2O2预处理后再进行紫外线照射，也会增强黑暗中和光修复处理后（6-4）PD的消失。H2O2预处理的增强程度与FL预照明的增强程度几乎相同。
