Hosoi K, Kurabuchi S, Kikkawa Y, Tada J, Akamatsu T, Yamanaka N, Matsumoto T, Kanamori N, Tsumura K
Dept. of Physiology, Tokushima University School of Dentistry, Japan.
Eur J Morphol. 1998 Aug;36 Suppl:82-5.
Four major enzymes of the tissue kallikrein family were purified from the mouse submandibular gland and characterized. The sequences indicated that they were mK1, mK9, mK13, and mK22. All four enzymes showed kinin-releasing activity, with mK1 exhibiting the highest activity. Like mK13, mK9 and mK22 also processed prorenin to give renin and/or arginyl renin, although their activities were less than that of mK13. The results suggest that tissue kallikrein family enzymes bearing higher kinin-releasing activity have lower prorenin-converting activity and vice versa. These enzymes may possibly have a physiological role in the tissue renin-angiotensin system.
从小鼠颌下腺中纯化并鉴定了组织激肽释放酶家族的四种主要酶。序列分析表明它们分别是mK1、mK9、mK13和mK22。这四种酶均具有激肽释放活性，其中mK1的活性最高。与mK13一样，mK9和mK22也能将血管紧张素原加工成肾素和/或精氨酰肾素，尽管它们的活性低于mK13。结果表明，激肽释放活性较高的组织激肽释放酶家族酶的血管紧张素原转化活性较低，反之亦然。这些酶可能在组织肾素-血管紧张素系统中发挥生理作用。
