Gavrilov V V, Wiener S I, Berthoz A
CNRS-Collège de France Laboratoire de Physiologie de la Perception et de l'Action UMR-C 9950, Paris, France.
Hippocampus. 1998;8(5):475-90. doi: 10.1002/(SICI)1098-1063(1998)8:5<475::AID-HIPO7>3.0.CO;2-H.
This study investigated location-, movement-, and directional-selectivity of action potential discharges of hippocampal neurons in awake rats subjected to passive displacements in order to estimate vestibular contributions to this activity. Water-deprived rats were habituated to being restrained in a sling mounted on a moving robot. The extracellular activity of single complex-spike cells in area CA1 of the hippocampus was recorded with glass micropipettes in the rats during passive translations, rotations, and immobility. The robot made a standardized series of trajectories starting from each of four corners of a square enclosure surrounded by black curtains. A drop of water was delivered to the rat each time the robot arrived at one designated corner of the arena. The activities of 29 neurons were investigated in 45 recording sessions (16 of which were in total darkness) in four rats. Hippocampal neurons recorded in 31 sessions (9 sessions in the dark) had significant location-selective increases or decreases in firing rate as the rat was passively displaced or immobile within the experimental arena. In 20 sessions (6 in the dark) direction-selective discharges were found when the rat was in the corners. In six sessions, cells discharged selectively during movement initiation or termination. These data suggest that information essential for path integration is present in the hippocampus and that inertial cues could play a vital role in hippocampal spatial functions. These results resemble those of O'Mara et al. ([1994] J Neurosci 14:6511) using the same protocol in macaques, suggesting similarities in hippocampal processing and function.
本研究调查了清醒大鼠在被动位移时海马神经元动作电位放电的位置、运动和方向选择性，以评估前庭对该活动的贡献。缺水大鼠习惯被限制在安装在移动机器人上的吊索中。在大鼠进行被动平移、旋转和静止时，用玻璃微电极记录海马CA1区单个复合棘波细胞的细胞外活动。机器人从被黑色窗帘包围的方形围栏的四个角中的每个角开始，进行一系列标准化轨迹运动。每次机器人到达竞技场的一个指定角落时，就给大鼠一滴水。在四只大鼠的45次记录实验（其中16次在完全黑暗中）中，研究了29个神经元的活动。在31次记录实验（9次在黑暗中）中记录的海马神经元，当大鼠在实验区域内被动位移或静止时，其放电频率有显著的位置选择性增加或减少。在20次记录实验（6次在黑暗中）中，当大鼠处于角落时发现了方向选择性放电。在6次记录实验中，细胞在运动开始或终止时选择性放电。这些数据表明，路径整合所需的信息存在于海马中，并且惯性线索可能在海马空间功能中起重要作用。这些结果与奥马拉等人（[1994]《神经科学杂志》14:6511）在猕猴中使用相同方案得到的结果相似，表明海马处理和功能存在相似性。
