Barthó L, Lénárd L, Szigeti R
Department of Pharmacology and Pharmacotherapy, University Medical School of Pécs, Hungary.
Naunyn Schmiedebergs Arch Pharmacol. 1998 Oct;358(4):496-9. doi: 10.1007/pl00005283.
The effect of the nitric oxide synthase inhibitor N(G)-nitro-L-arginine (L-NOARG; 100 microM) and the P2 purinoceptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS; 50 microM) was investigated on the non-adrenergic, non-cholinergic (NANC) relaxant response of the guinea-pig isolated taenia caeci to electrical field stimulation at 1 or 10 Hz, under isotonic recording conditions. Either drug alone caused an about 50% inhibition, while combining the two drugs nearly abolished the response at both frequencies. The inhibitory effect of L-NOARG (100 microM) was partly reversed by L-arginine (30 mM). PPADS, but not L-NOARG, inhibited the relaxant effect of exogenous ATP, but not that of the nitric oxide donor sodium nitroprusside. It is concluded that both nitric oxide and ATP are involved in the mediation of NANC relaxation in the taenia caeci, in an apparently additive manner.
在等张记录条件下，研究了一氧化氮合酶抑制剂N（G）-硝基-L-精氨酸（L-NOARG；100微摩尔）和P2嘌呤受体拮抗剂吡哆醛磷酸-6-偶氮苯基-2'，4'-二磺酸（PPADS；50微摩尔）对豚鼠离体盲肠带对1或10赫兹电场刺激的非肾上腺素能、非胆碱能（NANC）舒张反应的影响。单独使用任何一种药物均可引起约50%的抑制，而两种药物联合使用则几乎消除了两种频率下的反应。L-精氨酸（30毫摩尔）可部分逆转L-NOARG（100微摩尔）的抑制作用。PPADS可抑制外源性ATP的舒张作用，但不抑制一氧化氮供体硝普钠的舒张作用，而L-NOARG则无此作用。结论是，一氧化氮和ATP均以明显相加的方式参与盲肠带NANC舒张的介导。
