Bein G, Driller B, Schürmann M, Schneider P M, Kirchner H
Institute of Clinical Immunology and Transfusion Medicine, Justus-Liebig-University, Giessen, Germany.
Int J Legal Med. 1998;111(6):328-30. doi: 10.1007/s004140050181.
The investigation of a case of disputed paternity revealed indirect exclusion of the alleged father in the haptoglobin system and in the DNA single-locus system D16S309/Hinf I (MS205). The paternity index for the non-exclusion systems was > 10(6). Since both exclusion systems (HP and MS205) are located on chromosome 16, we investigated 10 microsatellite loci covering this chromosome with 10-20 cM resolution. Analysis of the child's chromosome showed only alleles of maternal origin and lack of inheritance of paternal alleles for five informative loci. The markers close to the centromere of chromosome 16 were heterozygous, whereas distal loci were either heterozygous or homozygous for maternal alleles. This is consistent with a maternal meiosis I nondisjunction of chromosome 16 leading to maternal uniparental heterodisomy. This case emphasizes that the opinion of non-paternity should be based on the absence of paternal alleles at genetic systems located on at least two different chromosomes.
对一例亲权纠纷案件的调查显示，在触珠蛋白系统和DNA单基因座系统D16S309/Hinf I（MS205）中，对被指控父亲存在间接排除。非排除系统的亲权指数>10(6)。由于两个排除系统（HP和MS205）都位于16号染色体上，我们研究了覆盖该染色体的10个微卫星基因座，分辨率为10 - 20 cM。对孩子染色体的分析显示，在5个信息性基因座上，只有母源等位基因，且缺乏父源等位基因的遗传。靠近16号染色体着丝粒的标记是杂合的，而远端基因座对于母源等位基因要么是杂合的，要么是纯合的。这与16号染色体的母源减数分裂I不分离导致母源单亲二体性是一致的。该案例强调，非父权认定的观点应基于至少两个不同染色体上的遗传系统中不存在父源等位基因。
