Belitser V A, Varetskaia T V, Tsynkalovskaia S N, Tsariuk L A, Shevchenko L I
Ukr Biokhim Zh. 1976 Jul-Aug;48(4):521-32.
A new diagnostic method to determine fibrinogen and fibrin splitting products is based on the fact that high-molecular fragments acting as specific inhibitors of fibrin self-assembly compose these products. Fibrinogen and fibrin splitting products are detected and determined quantitatively by prolongation of coagulation time in the test with the standard monomeric fibrin. The number of units of anticoagulating activity in the studied urine are the results of the determinations. Sensitivity of the method if necessary may be increased by adding of a certain amount of the fibrinogen and fibrin splitting products to the test that makes a favourable background for the action of the products. The method is rather sensitive, reliable and simple. It may be used in ordinary clinical laboratories. The method proved to be quite satisfactory when testing renal diseases at the clinic. It is of great significance for early detection of the transplanted kidney rejection. The new method is compared with the known immunological method which consist in determination of the inhibitory effect of the fibrinogen and fibrin splitting products on agglutination of erythrocytes of specific antibodies; the erythrocytes being subjected to "tanning" and "loading" with fibrinogen. A satisfactory correlation is found between the results of two methods.
一种用于测定纤维蛋白原和纤维蛋白降解产物的新诊断方法基于这样一个事实，即作为纤维蛋白自组装特异性抑制剂的高分子片段构成了这些产物。通过在标准单体纤维蛋白试验中延长凝血时间来检测和定量测定纤维蛋白原和纤维蛋白降解产物。所研究尿液中抗凝活性单位的数量即为测定结果。如有必要，可通过向试验中添加一定量的纤维蛋白原和纤维蛋白降解产物来提高该方法的灵敏度，这为产物的作用创造了有利背景。该方法相当灵敏、可靠且简单。它可用于普通临床实验室。在临床检测肾脏疾病时，该方法被证明相当令人满意。它对于早期检测移植肾排斥反应具有重要意义。将该新方法与已知的免疫方法进行了比较，后者在于测定纤维蛋白原和纤维蛋白降解产物对特异性抗体红细胞凝集的抑制作用；红细胞用纤维蛋白原进行“鞣酸处理”和“负载”。两种方法的结果之间发现了令人满意的相关性。
