CD44变体6和7在人子宫内膜癌中的过表达。

Overexpression of CD44 variants 6 and 7 in human endometrial cancer.

作者信息

Katsura M, Furumoto H, Nishimura M, Kamada M, Aono T

机构信息

Department of Obstetrics and Gynecology, University of Tokushima School of Medicine, 3-18-15 Kuramoto, Tokushima, 770, Japan.

出版信息

Gynecol Oncol. 1998 Nov;71(2):185-9. doi: 10.1006/gyno.1998.5169.

DOI:10.1006/gyno.1998.5169

PMID:9826458

Abstract

OBJECTIVES

The expression of CD44 V6, V7, and V10 in normal endometrium and endometrial cancer was compared.

METHODS

Using reverse transcription polymerase chain reaction (RT-PCR) blot analysis, the expression of mRNA containing CD44 V6, V7, and V10 was determined in 19 normal endometrium and 27 endometrial cancer samples. Immunohistochemical staining of CD44 V6 and V7 was performed in the same samples.

RESULTS

In RT-PCR analysis, the CD44 variant forms containing V6 and V7 exons were expressed in 96 and 93% of endometrial cancer tissues, respectively. These proportions were significantly higher than those in normal endometrium (V6, 63%; V7, 58%) (P < 0.01). CD44 V10 was expressed in 96% of endometrial cancers and 89% of normal endometrial samples. In immunohistochemical staining, CD44 V6 and V7 were detected in 48 and 61% of endometrial cancers and in 26 and 42% of normal endometrial samples, respectively. Neither of these differences was significant. No correlation was found between the expression of CD44 variants and any clinicopathological features.

CONCLUSION

CD44 V6 and V7 were expressed in a significantly larger proportion of endometrial cancers than normal endometrial samples. However, they were also expressed in a considerable proportion of normal endometria. These findings suggest that CD44 V6 and V7 play roles in normal endometrial function and overexpression of CD44 V6 and V7 is not related to the metastatic potential of endometrial cancer.

摘要

目的

比较CD44 V6、V7和V10在正常子宫内膜和子宫内膜癌中的表达。

方法

采用逆转录聚合酶链反应（RT-PCR）印迹分析，测定19例正常子宫内膜和27例子宫内膜癌样本中含CD44 V6、V7和V10的mRNA表达。对相同样本进行CD44 V6和V7的免疫组织化学染色。

结果

在RT-PCR分析中，含V6和V7外显子的CD44变异体形式分别在96%和93%的子宫内膜癌组织中表达。这些比例显著高于正常子宫内膜（V6，63%；V7，58%）（P<0.01）。CD44 V10在96%的子宫内膜癌和89%的正常子宫内膜样本中表达。在免疫组织化学染色中，CD44 V6和V7分别在48%和61%的子宫内膜癌以及26%和42%的正常子宫内膜样本中检测到。这些差异均无统计学意义。未发现CD44变异体的表达与任何临床病理特征之间存在相关性。