Silva E G, Tornos C, Deavers M, Kaisman K, Gray K, Gershenson D
Department of Pathology, Department of Veterinary Medicine, Department of Gynecologic Oncology, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, USA.
Gynecol Oncol. 1998 Nov;71(2):240-6. doi: 10.1006/gyno.1998.5153.
We have been searching for an animal model for ovarian epithelial neoplasms. Our previous study suggested that by giving intermediate doses of testosterone to guinea pigs it is possible to induce cystadenomas in the ovaries in 6 to 10 months.
In this study we investigated the effect of different estrogens including estradiol, diethylstilbestrol (DES), estrone, dienestrol, and hexestrol in 24 guinea pigs. Five guinea pigs received sterile water and were used as controls.
Bilateral serous cysts were seen in the ovaries of five guinea pigs that received low (0.25 and 0.35 mg) and intermediate (0.5 and 0.7 mg) doses of estradiol for 2 to 9 months. Surface papillary neoplasms were seen in the ovaries of four guinea pigs that received DES at an intermediate dose (6 mg) and high doses (10 and 12 mg) for 3 to 12 months. The ovaries of the other guinea pigs were unremarkable. Estradiol was measured by radioimmunoassay in three guinea pigs. In the serum it ranged from 450 to 580 pg/ml, and in the ovarian cystadenomas it ranged from 1020 to 6575 pg/ml.
(1) It is possible to induce ovarian neoplasms using estrogenic hormones. (2) The best results are obtained with intermediate doses of estradiol and DES. (3) These two hormones induce different lesions: estradiol is associated with cystic lesions and DES with surface papillary tumors. These observations, together with our previous finding that testosterone induces neoplasms with a predominantly glandular pattern, establish the guinea pig as an excellent animal to study ovarian neoplasms, confirm the significance of hormones in the development of ovarian epithelial tumors, and suggest that the different histologic appearance of ovarian epithelial neoplasms might be related to different hormonal effects.
我们一直在寻找卵巢上皮性肿瘤的动物模型。我们之前的研究表明，给豚鼠注射中等剂量的睾酮，有可能在6至10个月内诱导卵巢发生囊腺瘤。
在本研究中，我们调查了不同雌激素（包括雌二醇、己烯雌酚、雌酮、二烯雌酚和己烷雌酚）对24只豚鼠的影响。五只豚鼠接受无菌水，用作对照。
接受低剂量（0.25和0.35毫克）和中等剂量（0.5和0.7毫克）雌二醇注射2至9个月的五只豚鼠卵巢中出现双侧浆液性囊肿。接受中等剂量（6毫克）和高剂量（10和12毫克）己烯雌酚注射3至12个月的四只豚鼠卵巢中出现表面乳头状肿瘤。其他豚鼠的卵巢未见异常。通过放射免疫分析法对三只豚鼠的雌二醇进行了测量。血清中雌二醇水平为450至580皮克/毫升，卵巢囊腺瘤中雌二醇水平为1020至6575皮克/毫升。
（1）使用雌激素有可能诱导卵巢肿瘤。（2）中等剂量的雌二醇和己烯雌酚效果最佳。（3）这两种激素诱导不同的病变：雌二醇与囊性病变有关，己烯雌酚与表面乳头状肿瘤有关。这些观察结果，连同我们之前发现睾酮诱导的肿瘤主要为腺性模式，确立了豚鼠作为研究卵巢肿瘤的优良动物，证实了激素在卵巢上皮性肿瘤发生中的重要性，并表明卵巢上皮性肿瘤不同的组织学表现可能与不同的激素作用有关。
