Misao R, Sun W S, Iwagaki S, Fujimoto J, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
Biochem Biophys Res Commun. 1998 Nov 18;252(2):302-6. doi: 10.1006/bbrc.1998.9642.
We demonstrated the expression of various exon-deleted progesterone receptor (PR) variant mRNAs in human uterine endometrium and ovarian endometriosis using the reverse transcription-polymerase chain reaction-DNA sequencing analyses. In addition to PR wild-type mRNA, various exon-deleted PR variant mRNAs were identified in all samples analyzed. The sequence of these variants showed a perfect junction between exons surrouding the deletion area. PR wild-type, exon 6-deleted, exon 4-deleted, exon 5, 6-deleted and exon 4, 5, 6-deleted PR variant mRNAs were observed in all samples analyzed. Exon 4, 6-deleted PR mRNA was observed only in ovarian endometriosis. This is the first study to demonstrate the coexpression of various PR exon-deleted variant mRNAs with the wild-type in uterine endometrium and ovarian endometriosis. All resulting variant proteins might indicate functional diversity and modify the progestational action of wild-type PR, and thus be involved in the pathophysiology of ovarian endometriosis.
我们通过逆转录-聚合酶链反应- DNA测序分析，证实了人子宫内膜和卵巢子宫内膜异位症中各种外显子缺失的孕酮受体（PR）变异体mRNA的表达。除了PR野生型mRNA外，在所有分析样本中均鉴定出各种外显子缺失的PR变异体mRNA。这些变异体的序列在缺失区域周围的外显子之间显示出完美的连接。在所有分析样本中均观察到PR野生型、外显子6缺失、外显子4缺失、外显子5、6缺失和外显子4、5、6缺失的PR变异体mRNA。外显子4、6缺失的PR mRNA仅在卵巢子宫内膜异位症中观察到。这是首次证明各种PR外显子缺失变异体mRNA与野生型在子宫内膜和卵巢子宫内膜异位症中共表达的研究。所有产生的变异蛋白可能表明功能多样性，并改变野生型PR的孕激素作用，从而参与卵巢子宫内膜异位症的病理生理过程。
