• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Millimolar concentrations of zinc and other metal cations cause sedimentation of DNA.毫摩尔浓度的锌和其他金属阳离子会导致DNA沉淀。
Nucleic Acids Res. 1998 Dec 1;26(23):5295-9. doi: 10.1093/nar/26.23.5295.
2
pH-dependent sedimentation of DNA in the presence of divalent, but not monovalent, metal ions.在存在二价而非一价金属离子的情况下，DNA 随 pH 值的沉淀。
Anal Biochem. 2021 Mar 1;616:114099. doi: 10.1016/j.ab.2020.114099. Epub 2020 Dec 31.
3
A gel electrophoresis study of the competitive effects of monovalent counterion on the extent of divalent counterions binding to DNA.一项关于单价抗衡离子对二价抗衡离子与DNA结合程度的竞争效应的凝胶电泳研究。
Biophys J. 1998 Feb;74(2 Pt 1):964-73. doi: 10.1016/S0006-3495(98)74019-6.
4
Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd.DNA-金属络合物的拉曼光谱。I. 二价阳离子：镁、钙、锶、钡、锰、钴、镍、铜、钯和镉的相互作用及构象效应
Biophys J. 1993 Nov;65(5):1916-28. doi: 10.1016/S0006-3495(93)81263-3.
5
'Footprinting' proteins on DNA with peroxonitrous acid.用过氧亚硝酸对DNA上的蛋白质进行“足迹”分析
Nucleic Acids Res. 1993 May 25;21(10):2473-8. doi: 10.1093/nar/21.10.2473.
6
The effect of manipulating sediment pH on the porewater chemistry of copper- and zinc-spiked sediments.调控沉积物pH值对添加铜和锌的沉积物孔隙水化学性质的影响。
Chemosphere. 2007 Oct;69(7):1089-99. doi: 10.1016/j.chemosphere.2007.04.029. Epub 2007 Jun 18.
7
Phosphate uptake behavior of ZnAlZr ternary layered double hydroxides through surface precipitation.通过表面沉淀法研究 ZnAlZr 三元层状双氢氧化物的磷酸盐吸附行为。
J Colloid Interface Sci. 2010 Jan 15;341(2):289-97. doi: 10.1016/j.jcis.2009.09.059. Epub 2009 Oct 2.
8
The binding of zinc (II) to a double-stranded oligodeoxyribonucleotide. A voltammetric study.
Biophys Chem. 2004 Dec 1;112(1):59-67. doi: 10.1016/j.bpc.2004.07.003.
9
Influence of pH and inorganic phosphate on toxicity of zinc to Arthrobacter sp. isolated from heavy-metal-contaminated sediments.pH 值和无机磷酸盐对从重金属污染沉积物中分离的节杆菌属菌株毒性的影响。
Environ Sci Technol. 2010 Oct 1;44(19):7302-8. doi: 10.1021/es100117f.
10
Inhibitory effect of divalent metal cations on zinc uptake via mouse Zrt-/Irt-like protein 8 (ZIP8).二价金属阳离子对小鼠 Zrt-/Irt 样蛋白 8（ZIP8）介导的锌摄取的抑制作用。
Life Sci. 2017 Mar 15;173:80-85. doi: 10.1016/j.lfs.2016.12.006. Epub 2016 Dec 10.

引用本文的文献

1
pH-dependent sedimentation of DNA in the presence of divalent, but not monovalent, metal ions.在存在二价而非一价金属离子的情况下，DNA 随 pH 值的沉淀。
Anal Biochem. 2021 Mar 1;616:114099. doi: 10.1016/j.ab.2020.114099. Epub 2020 Dec 31.
2
Zn2+ blocks annealing of complementary single-stranded DNA in a sequence-selective manner.锌离子以序列选择性的方式阻断互补单链DNA的退火过程。
Sci Rep. 2014 Jun 26;4:5464. doi: 10.1038/srep05464.
3
Nucleic acid-lipid membrane interactions studied by DSC.通过差示扫描量热法研究核酸与脂质膜的相互作用。
J Pharm Bioallied Sci. 2011 Jan;3(1):70-6. doi: 10.4103/0975-7406.76470.
4
Characterization and structure of the manganese-responsive transcriptional regulator ScaR.锰响应转录调节因子ScaR的表征与结构
Biochemistry. 2009 Nov 3;48(43):10308-20. doi: 10.1021/bi900980g.
5
Nucleic acids bind to nanoparticulate iron (II) monosulphide in aqueous solutions.核酸在水溶液中与纳米颗粒状硫化亚铁（II）结合。
Orig Life Evol Biosph. 2008 Jun;38(3):257-70. doi: 10.1007/s11084-008-9132-7. Epub 2008 Apr 13.

毫摩尔浓度的锌和其他金属阳离子会导致DNA沉淀。

Millimolar concentrations of zinc and other metal cations cause sedimentation of DNA.

作者信息

Kejnovsky E, Kypr J

机构信息

Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265 Brno, Czech Republic.

出版信息

Nucleic Acids Res. 1998 Dec 1;26(23):5295-9. doi: 10.1093/nar/26.23.5295.

DOI:10.1093/nar/26.23.5295
PMID:9826751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC148012/
Abstract

We demonstrate that DNA sediments in the presence of millimolar concentrations of zinc or related metal cations and that EDTA entirely dissolves the sediment. The sedimentation is promoted by alkaline pH but the pH dependence is abolished by submillimolar concentrations of phosphate anions. We suspect that the metal cations generate sedimenting particles of insoluble hydroxides or phosphates for which DNA has a strong affinity. The events involved in DNA-metal phosphate co-sedimentation are similar to the processes that enable calcium phosphate-assisted transfection. Hence, work with even submillimolar concentrations of zinc and most other metal cations, which many DNA-binding proteins need for their activities, requires care to avoid the sedimentation of DNA. Literature reporting about zinc effects on DNA is discussed from the point of view of the present results.

摘要

我们证明，在存在毫摩尔浓度的锌或相关金属阳离子的情况下，DNA会发生沉淀，而EDTA能完全溶解该沉淀物。碱性pH会促进沉淀，但亚毫摩尔浓度的磷酸根阴离子会消除对pH的依赖性。我们怀疑金属阳离子会生成DNA具有强烈亲和力的不溶性氢氧化物或磷酸盐沉淀颗粒。DNA-金属磷酸盐共沉淀所涉及的过程类似于使磷酸钙辅助转染成为可能的过程。因此，即使是许多DNA结合蛋白发挥活性所需的亚毫摩尔浓度的锌和大多数其他金属阳离子，在使用时也需要小心避免DNA沉淀。从当前结果的角度讨论了有关锌对DNA影响的文献报道。