Fecteau K A, Eiler H
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37996-4500, USA.
In Vivo. 1998 Sep-Oct;12(5):485-8.
The chemotherapeutic antibiotic doxorubicin (Adriamycin) was reported to inhibit bacterial collagenase activity when tested on both a synthetic substrate and intact collagen. Our objective was to establish whether doxorubicin would inhibit bacterial collagenase activity on a bovine placentome model. Metabolically active, isolated bovine placentomes were infused, via umbilical vessels, with a mixture of bacterial collagenase and doxorubicin. Six experimental groups were used. Group (1) comprised of saline controls; (2), collagenase at 1,200 U/cc; (3, 4, and 5), collagenase (1,200 U/cc) plus doxorubicin at 0.04 mg/cc, 0.02 mg/cc, 0.01 mg/cc, respectively; (6), doxorubicin at 0.02 mg/cc. After 5 hrs of incubation (39 degrees C), manometric pressure (needed to separate caruncle and cotyledon), caruncle-cotyledon interface hydroxyproline (collagenolysis), and total protein (proteolysis) were determined. Results indicated no (P > 0.01) inhibition of collagenase by doxorubicin. We concluded that doxorubicin (at above dosages) is not an inhibitor for bacterial collagenase when tested on bovine placentomes.
据报道，化疗抗生素阿霉素（ Adriamycin ）在合成底物和完整胶原蛋白上进行测试时，可抑制细菌胶原酶活性。我们的目的是确定阿霉素在牛胎盘模型上是否会抑制细菌胶原酶活性。通过脐血管向代谢活跃的离体牛胎盘注入细菌胶原酶和阿霉素的混合物。使用了六个实验组。第（ 1 ）组为生理盐水对照组；第（ 2 ）组，胶原酶浓度为 1200 U/cc ；第（ 3 、 4 和 5 ）组，胶原酶（ 1200 U/cc ）分别加阿霉素 0.04 mg/cc 、 0.02 mg/cc 、 0.01 mg/cc ；第（ 6 ）组，阿霉素浓度为 0.02 mg/cc 。在 39 摄氏度孵育 5 小时后，测定压力（分离肉阜和子叶所需的压力）、肉阜 - 子叶界面羟脯氨酸（胶原溶解）和总蛋白（蛋白水解）。结果表明阿霉素对胶原酶无抑制作用（ P > 0.01 ）。我们得出结论，在牛胎盘上进行测试时，阿霉素（上述剂量）不是细菌胶原酶的抑制剂。
