Kanda Y, Tanaka Y, Shirakawa K, Yatomi T, Nakamura N, Kami M, Saito T, Izutsu K, Asai T, Yuji K, Ogawa S, Honda H, Mitani K, Chiba S, Yazaki Y, Hirai H
Department of Cell Therapy and Transplantation Medicine, Faculty of Medicine, University of Tokyo, Japan.
Bone Marrow Transplant. 1998 Oct;22(8):751-4. doi: 10.1038/sj.bmt.1701427.
Acute graft-versus-host disease (aGVHD) is a major complication following allogeneic bone marrow transplantation (BMT). Recently, accumulating evidence indicates that the Fas/Fas ligand (FasL) system is implicated in the pathogenesis of aGVHD in murine models. We determined the serum levels of soluble FasL (sFasL) in BMT recipients using an enzyme-linked immunosorbent assay. The serum sFasL was suppressed during the period of myelosuppression following the preparative regimen and subsequently increased with hematopoietic reconstitution after BMT. In patients with aGVHD, the serum sFasL level was significantly higher than in those without aGVHD. In the mixed lymphocyte reaction assay, sFasL in the supernatants was increased with a significant correlation to the level of 3H-thymidine uptake. Our findings suggest that the Fas/FasL system is activated by allogeneic stimulation and may have close correlation to the development of aGVHD in human BMT.
急性移植物抗宿主病（aGVHD）是异基因骨髓移植（BMT）后的一种主要并发症。最近，越来越多的证据表明，Fas/Fas配体（FasL）系统与小鼠模型中aGVHD的发病机制有关。我们使用酶联免疫吸附测定法测定了BMT受者血清中可溶性FasL（sFasL）的水平。在预处理方案后的骨髓抑制期，血清sFasL受到抑制，随后在BMT后随着造血重建而升高。在患有aGVHD的患者中，血清sFasL水平显著高于未患aGVHD的患者。在混合淋巴细胞反应试验中，上清液中的sFasL增加，与3H-胸腺嘧啶核苷摄取水平显著相关。我们的研究结果表明，Fas/FasL系统被同种异体刺激激活，可能与人类BMT中aGVHD的发生密切相关。
