Nunan L M, Poulos B T, Lightner D V
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA.
Dis Aquat Organ. 1998 Oct 8;34(2):87-91. doi: 10.3354/dao034087.
Taura Syndrome Virus (TSV) has adversely affected the shrimp culture industries of the Americas. First recognized in 1992, this viral agent has spread throughout the shrimp growing regions of South and Central America to become established in North America in the short span of 5 yr. Diagnostic methods for TSV include histopathology, bioassay using susceptible Penaeus vannamei as the indicator species and in situ hybridization with TSV specific complimentary DNA (cDNA) gene probes. An additional method for detecting TSV is through the use of reverse transcription polymerase chain reaction (RT-PCR). Two oligonucleotide primers were selected using the sequence information from a cloned cDNA segment of the TSV genome. The primers, designated 9195 and 9992, used in the RT-PCR procedure amplify a 231 base pair (bp) fragment of the cDNA. Using the RT-PCR technique, TSV has been detected in the hemolymph of P. stylirostris and P. vannamei with experimentally induced TSV infections.
桃拉综合征病毒（TSV）对美洲的对虾养殖业产生了不利影响。该病毒于1992年首次被发现，在短短5年内已传播至南美洲和中美洲的对虾养殖区，并在北美洲定殖。TSV的诊断方法包括组织病理学、以易感的南美白对虾作为指示物种的生物测定以及用TSV特异性互补DNA（cDNA）基因探针进行原位杂交。检测TSV的另一种方法是使用逆转录聚合酶链反应（RT-PCR）。利用从TSV基因组的一个克隆cDNA片段获得的序列信息选择了两条寡核苷酸引物。在RT-PCR程序中使用的引物，命名为9195和9992，可扩增出一个231碱基对（bp）的cDNA片段。利用RT-PCR技术，在实验性感染TSV的斯氏对虾和南美白对虾的血淋巴中检测到了TSV。
