根瘤菌新属——水栖共生根瘤菌及新种，能在塞内加尔高效结瘤水生海漂豆的固氮细菌。

Allorhizobium undicola gen. nov., sp. nov., nitrogen-fixing bacteria that efficiently nodulate Neptunia natans in Senegal.

作者信息

de Lajudie P, Laurent-Fulele E, Willems A, Torck U, Coopman R, Collins M D, Kersters K, Dreyfus B, Gillis M

机构信息

Laboratoire de Microbiologie des Sols, Dakar, Senegal, West Africa.

出版信息

Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1277-90. doi: 10.1099/00207713-48-4-1277.

DOI:10.1099/00207713-48-4-1277

PMID:9828428

Abstract

A group of nodule isolates from Neptunia natans, an indigenous stemnodulated tropical legume found in waterlogged areas of Senegal, was studied. Polyphasic taxonomy was performed, including SDS-PAGE of total proteins, auxanography using API galleries, host-plant specificity, PCR-RFLP of the internal transcribed spacer region between the 16S and the 23S rRNA coding genes, 16S rRNA gene sequencing and DNA-DNA hybridization. It was demonstrated that this group is phenotypically and phylogenetically separate from the known species of Rhizobium, Sinorhizobium, Mesorhizobium, Agrobacterium, Bradyrhizobium and Azorhizobium. Its closest phylogenetic neighbour, as deduced by 16S rRNA gene sequencing, is Agrobacterium vitis (96.2% sequence homology). The name Allorhizobium undicola gen. nov., sp. nov., is proposed for this group of bacteria, which are capable of efficient nitrogen-fixing symbiosis with Neptunia natans, and the type strain is ORS 992T (= LMG 11875T).

摘要

对从 Neptunia natans（一种原产于塞内加尔涝渍地区的茎瘤热带豆科植物）分离得到的一组根瘤菌进行了研究。采用了多相分类法，包括总蛋白的 SDS-PAGE、使用 API 鉴定条的营养谱分析、宿主植物特异性、16S 和 23S rRNA 编码基因之间的内转录间隔区的 PCR-RFLP、16S rRNA 基因测序以及 DNA-DNA 杂交。结果表明，该菌群在表型和系统发育上与已知的根瘤菌属、中华根瘤菌属、中慢生根瘤菌属、土壤杆菌属、慢生根瘤菌属和固氮根瘤菌属物种不同。根据 16S rRNA 基因测序推断，其最接近的系统发育邻居是葡萄土壤杆菌（序列同源性为 96.2%）。建议将这组能够与 Neptunia natans 高效共生固氮的细菌命名为 Allorhizobium undicola 新属，新种，其模式菌株为 ORS 992T（= LMG 11875T）。