Faunt K K, Jones B D, Turk J R, Cohn L A, Dodam J R
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
Am J Vet Res. 1998 Nov;59(11):1499-502.
To determine whether lung biopsy specimens obtained during thoracoscopy, using a commercially available ligature, can provide an adequate amount of tissue for histologic evaluation and to characterize changes in the lungs and thoracic cavity that result from the procedure.
6 mixed-breed dogs.
All dogs underwent 2 anesthetic episodes. The first anesthetic episode was a sham procedure. During the second anesthetic episode, each dog underwent a thoracoscopic procedure to obtain a lung biopsy specimen, using a commercially available ligature. Biopsy specimens were assessed subjectively by means of histologic evaluation. Samples for arterial blood gas analysis were obtained, and thoracic radiography was performed after surgery. Dogs were evaluated daily for 14 days after thoracoscopy and then were euthanatized. Tissues were evaluated grossly and histologically.
Excellent intraoperative visibility and biopsy specimens adequate for histologic evaluation were obtained from all dogs. Significant differences were not found between arterial blood gas values of sham- and thoracoscopy-treated dogs for samples obtained 0.25, 2, and 24 hours after extubation. Examination of thoracic radiographs obtained 2 and 24 hours after thoracoscopy revealed minimal localized pathologic changes. All dogs were clinically normal 24 hours after thoracoscopy, and major postoperative complications were not detected. Gross and histologic findings of specimens obtained during necropsy revealed changes localized to biopsy and trocar sites.
Thoracoscopic placement of ligatures allowed procurement of lung lobe biopsy specimens from clinically normal dogs without complications.
This procedure may provide a safe and minimally invasive means of obtaining lung biopsy specimens from clinically affected dogs.
确定在胸腔镜检查过程中使用市售结扎线获取的肺活检标本是否能提供足够的组织用于组织学评估，并描述该操作导致的肺和胸腔的变化。
6只杂种犬。
所有犬均经历2次麻醉过程。第一次麻醉过程为假手术。在第二次麻醉过程中，每只犬均接受胸腔镜检查，使用市售结扎线获取肺活检标本。通过组织学评估对活检标本进行主观评估。采集动脉血气分析样本，并在术后进行胸部X线摄影。胸腔镜检查后对犬进行14天的每日评估，然后实施安乐死。对组织进行大体和组织学评估。
所有犬均获得了良好的术中视野和足以进行组织学评估的活检标本。拔管后0.25、2和24小时获取的样本，假手术组和胸腔镜检查组犬的动脉血气值之间未发现显著差异。胸腔镜检查后2和24小时的胸部X线片检查显示局部病理变化极小。所有犬在胸腔镜检查后24小时临床正常，未检测到主要的术后并发症。尸检时获取的标本的大体和组织学检查结果显示，变化局限于活检和套管针穿刺部位。
胸腔镜下放置结扎线可从临床正常犬获取肺叶活检标本，且无并发症。
该操作可为从临床患病犬获取肺活检标本提供一种安全且微创的方法。
