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相似文献

1
secG and temperature modulate expression of azide-resistant and signal sequence suppressor phenotypes of Escherichia coli secA mutants.secG和温度调节大肠杆菌secA突变体的叠氮化物抗性和信号序列抑制子表型的表达。
J Bacteriol. 1998 Dec;180(23):6419-23. doi: 10.1128/JB.180.23.6419-6423.1998.
2
Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA.大肠杆菌中信号序列缺陷和叠氮化物抗性的抑制通常源于secA基因的相同突变。
J Bacteriol. 1995 Jun;177(12):3518-26. doi: 10.1128/jb.177.12.3518-3526.1995.
3
Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.大肠杆菌的叠氮化物抗性突变体改变了SecA蛋白，SecA蛋白是蛋白质输出机制中对叠氮化物敏感的一个组分。
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8227-31. doi: 10.1073/pnas.87.21.8227.
4
Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene.大肠杆菌中赋予叠氮化物抗性的突变主要发生在secA基因中。
J Bacteriol. 1990 Nov;172(11):6607-10. doi: 10.1128/jb.172.11.6607-6610.1990.
5
Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations.SecA 同二聚体的核苷酸结合活性受温度的构象调节，并因 prlD 和 azi 突变而改变。
J Biol Chem. 2000 May 19;275(20):15440-8. doi: 10.1074/jbc.M000605200.
6
Roles of SecG in ATP- and SecA-dependent protein translocation.SecG在ATP和SecA依赖性蛋白质转运中的作用。
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13567-72. doi: 10.1073/pnas.95.23.13567.
7
Coupled structure changes of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and delta secG::kan double mutant.secAcsR11和delta secG::kan双突变体的合成致死性揭示的SecA和SecG的耦合结构变化。
Mol Microbiol. 1998 Jul;29(1):331-41. doi: 10.1046/j.1365-2958.1998.00937.x.
8
Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant.酸性磷脂含量的增加特异性地恢复了冷敏感型secA或secG缺失突变体中的蛋白质转运。
J Biol Chem. 1999 Oct 22;274(43):31020-4. doi: 10.1074/jbc.274.43.31020.
9
A novel class of secA alleles that exert a signal-sequence-dependent effect on protein export in Escherichia coli.一类新型的secA等位基因，其对大肠杆菌中的蛋白质输出发挥信号序列依赖性效应。
Genetics. 2002 Nov;162(3):1031-43. doi: 10.1093/genetics/162.3.1031.
10
SecYEG and SecA are the stoichiometric components of preprotein translocase.SecYEG和SecA是前体蛋白转运酶的化学计量组分。
J Biol Chem. 1995 Aug 25;270(34):20106-11. doi: 10.1074/jbc.270.34.20106.

引用本文的文献

1
The variable subdomain of Escherichia coli SecA functions to regulate SecA ATPase activity and ADP release.大肠杆菌 SecA 的可变亚结构域可调节 SecA ATP 酶活性和 ADP 释放。
J Bacteriol. 2012 May;194(9):2205-13. doi: 10.1128/JB.00039-12. Epub 2012 Mar 2.
2
Mapping of the SecA·SecY and SecA·SecG interfaces by site-directed in vivo photocross-linking.通过体内定向光交联映射 SecA·SecY 和 SecA·SecG 界面。
J Biol Chem. 2011 Apr 8;286(14):12371-80. doi: 10.1074/jbc.M110.182931. Epub 2011 Feb 11.
3
Probing the affinity of SecA for signal peptide in different environments.探究不同环境下SecA对信号肽的亲和力。
Biochemistry. 2005 Oct 25;44(42):13987-96. doi: 10.1021/bi050882k.
4
A novel class of secA alleles that exert a signal-sequence-dependent effect on protein export in Escherichia coli.一类新型的secA等位基因，其对大肠杆菌中的蛋白质输出发挥信号序列依赖性效应。
Genetics. 2002 Nov;162(3):1031-43. doi: 10.1093/genetics/162.3.1031.
5
The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.PrlA和PrlG表型是由转运体SecYEG亚基之间的结合松弛引起的。
EMBO J. 1999 Jun 15;18(12):3263-70. doi: 10.1093/emboj/18.12.3263.

本文引用的文献

1
The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events.大肠杆菌SecA ATP酶的催化循环包括两个不同的前体蛋白转运事件。
EMBO J. 1997 Dec 15;16(24):7297-304. doi: 10.1093/emboj/16.24.7297.
2
Interaction of SecB with soluble SecA.SecB与可溶性SecA的相互作用。
FEBS Lett. 1997 Oct 13;416(1):35-8. doi: 10.1016/s0014-5793(97)01142-3.
3
SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane.SecY和SecA相互作用，使SecA插入并使蛋白质穿过大肠杆菌质膜进行转运。
EMBO J. 1997 Nov 3;16(21):6384-93. doi: 10.1093/emboj/16.21.6384.
4
The chemistry and enzymology of the type I signal peptidases.I型信号肽酶的化学与酶学
Protein Sci. 1997 Jun;6(6):1129-38. doi: 10.1002/pro.5560060601.
5
Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.前体蛋白转运酶全酶的SecYE、SecG和SecDFyajC亚基的不同催化作用。
EMBO J. 1997 May 15;16(10):2756-68. doi: 10.1093/emboj/16.10.2756.
6
Identification of a region of interaction between Escherichia coli SecA and SecY proteins.大肠杆菌SecA蛋白与SecY蛋白之间相互作用区域的鉴定。
J Biol Chem. 1997 Apr 25;272(17):11302-6. doi: 10.1074/jbc.272.17.11302.
7
Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins.SecB与核糖体结合的多肽的结合特性与它与全长变性蛋白的结合特性相同。
Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):802-7. doi: 10.1073/pnas.94.3.802.
8
A significant fraction of functional SecA is permanently embedded in the membrane. SecA cycling on and off the membrane is not essential during protein translocation.相当一部分功能性SecA永久性地嵌入膜中。在蛋白质转运过程中，SecA在膜上的循环并非必不可少。
J Biol Chem. 1996 Nov 22;271(47):29698-706. doi: 10.1074/jbc.271.47.29698.
9
Escherichia coli preprotein translocase.大肠杆菌前体蛋白转运酶
J Biol Chem. 1996 Nov 22;271(47):29514-6. doi: 10.1074/jbc.271.47.29514.
10
Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.蛋白质转运所需配体诱导的Sec61p复合物寡聚环。
Cell. 1996 Nov 15;87(4):721-32. doi: 10.1016/s0092-8674(00)81391-4.

secG和温度调节大肠杆菌secA突变体的叠氮化物抗性和信号序列抑制子表型的表达。

secG and temperature modulate expression of azide-resistant and signal sequence suppressor phenotypes of Escherichia coli secA mutants.

作者信息

Ramamurthy V, Dapíc V, Oliver D

机构信息

Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06459, USA.

出版信息

J Bacteriol. 1998 Dec;180(23):6419-23. doi: 10.1128/JB.180.23.6419-6423.1998.

DOI:10.1128/JB.180.23.6419-6423.1998
PMID:9829959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107736/
Abstract

SecA is a dynamic protein that undergoes ATP-dependent membrane cycling to drive protein translocation across the Escherichia coli inner membrane. To understand more about this process, azide-resistant (azi) and signal sequence suppressor (prlD) alleles of secA were studied. We found that azide resistance is cold sensitive because of a direct effect on protein export, suggesting that SecA-membrane interaction is regulated by an endothermic step that is azide inhibitable. secG function is required for expression of azide-resistant and signal sequence suppressor activities of azi and prlD alleles, and in turn, these alleles suppress cold-sensitive and export-defective phenotypes of a secG null mutant. These remarkable genetic observations support biochemical data indicating that SecG promotes SecA membrane cycling and that this process is dependent on an endothermic change in SecA conformation.

摘要

SecA是一种动态蛋白，它通过ATP依赖的膜循环来驱动蛋白质穿过大肠杆菌内膜进行转运。为了更深入了解这一过程，我们研究了secA的叠氮化物抗性（azi）和信号序列抑制子（prlD）等位基因。我们发现叠氮化物抗性对温度敏感，因为它直接影响蛋白质输出，这表明SecA与膜的相互作用受一个可被叠氮化物抑制的吸热步骤调控。secG的功能对于azi和prlD等位基因的叠氮化物抗性及信号序列抑制子活性的表达是必需的，反过来，这些等位基因可抑制secG缺失突变体的温度敏感型和输出缺陷型表型。这些显著的遗传学观察结果支持了生化数据，这些数据表明SecG促进SecA膜循环，且这一过程依赖于SecA构象的吸热变化。