Elasri M O, Miller R V
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078, USA.
Appl Microbiol Biotechnol. 1998 Oct;50(4):455-8. doi: 10.1007/s002530051320.
We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisheri lux operon. This recA-lux fusion (pMOE15) was introduced into wild-type P. aeruginosa strain FRD1 and recA expression was monitored by measuring 490-nm light production. The RM4440 strain responded to increasing doses of ultraviolet radiation by an increase in its bioluminescence. RM4440 has the potential to be useful as a biosensor for the presence of DNA-damaging agents in the environment.
我们将铜绿假单胞菌recA启动子与无启动子的费氏弧菌lux操纵子融合。这种recA-lux融合体（pMOE15）被导入野生型铜绿假单胞菌菌株FRD1，并通过测量490纳米的发光来监测recA的表达。RM4440菌株对不断增加剂量的紫外线辐射的反应是生物发光增强。RM4440有潜力用作检测环境中DNA损伤剂存在的生物传感器。
