Janssens de Varebeke S, Goubau P, Beuselinck H K, Somers T h, Offeciers F E, Govaerts P J
University Department Otolaryngology, St-Augustinus Hospital, Antwerp-Wilrijk, Belgium.
Am J Otol. 1998 Nov;19(6):704-8.
To evaluate the potential risk of human immunodeficiency virus (HIV) transmission by tympano-ossicular allografts by studying the efficacy of standard preservation techniques to eliminate the presence of proviral HIV-1 DNA fragments in contaminated ossicles.
Randomized single-blind prospective study on the ossicles of HIV-1 patients.
Ossicles of five patients who had died of acquired immune deficiency syndrome (AIDS) (HIV-1 infection) were taken within 6 hours postmortem and allocated randomly to a treatment and nontreatment group. Liver and skin biopsies were taken as positive control specimens. PROCESSING: The treatment group was processed with standard techniques (formaldehyde) for tympano-ossicular allograft preservation and the nontreatment group was only washed, dried, and stored in sterile tubes at -700 degrees without further processing.
Proviral HIV-1 DNA was detected using polymerase chain reaction amplification techniques.
No proviral HIV-1 DNA was detected in any of the treated ossicles, whereas three of five sets of untreated ossicles were positive. The positive control specimens of all treated and nontreated sets were positive for proviral HIV-1 DNA.
These results suggest that the preservation technique for tympano-ossicular allografts is safe with regard to HIV-1 transmission.
通过研究标准保存技术消除受污染听骨中前病毒HIV-1 DNA片段存在的效果，评估鼓室听骨同种异体移植传播人类免疫缺陷病毒（HIV）的潜在风险。
对HIV-1患者的听骨进行随机单盲前瞻性研究。
从5例死于获得性免疫缺陷综合征（AIDS）（HIV-1感染）的患者尸检后6小时内获取听骨，并随机分配至治疗组和非治疗组。取肝脏和皮肤活检组织作为阳性对照标本。
治疗组采用标准技术（甲醛）处理以保存鼓室听骨同种异体移植，非治疗组仅进行冲洗、干燥，然后保存在-70℃无菌管中，不做进一步处理。
采用聚合酶链反应扩增技术检测前病毒HIV-1 DNA。
所有处理后的听骨均未检测到前病毒HIV-1 DNA，而五组未处理的听骨中有三组呈阳性。所有处理组和未处理组的阳性对照标本前病毒HIV-1 DNA均呈阳性。
这些结果表明，鼓室听骨同种异体移植的保存技术在HIV-1传播方面是安全的。
