大鼠和人肝脏小样本中戊巴比妥羟基化动力学
Amylobarbitone hydroxylation kinetics in small samples of rat and human liver.
作者信息
Fraser H S, Williams F M, Davies D L, Draffan G H, Davies D S
出版信息
Xenobiotica. 1976 Aug;6(8):465-72. doi: 10.3109/00498257609151659.
PMID:983119
Abstract
- Two micro methods for determining amylobarbitone hydroxylase activity from less than 3 mg liver are described. One is based on single-ion monitoring g.l.c.-mass spectrometry and the other on t.l.c. separation of 14C-labelled product. 2. Km and Vmax have been determined with rat liver and both needle and open biopsy samples of human liver. 3. Both methods are sufficiently sensitive and reproducible for use with 20 mg needle biopsies.
摘要
- 描述了两种从少于3毫克肝脏中测定异戊巴比妥羟化酶活性的微量方法。一种基于单离子监测气相色谱 - 质谱联用技术，另一种基于对14C标记产物的薄层层析分离。2. 已经用大鼠肝脏以及人肝脏的针刺活检和开放式活检样本测定了米氏常数（Km）和最大反应速度（Vmax）。3. 这两种方法对于20毫克的针刺活检样本而言都具有足够的灵敏度和可重复性。
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