Aniline metabolism in two species of Arachnida: water-soluble metabolites.

1. The metabolism of [14C]aniline in the cattle tick, Boophilus microplus (Canestrini) at 40 and 300 mug per animal) and in the spider, Nephila plumipes (at 40 mug per 100 mg) were studied. 2. In both species hydroxylation occurred yielding both free and conjugated o-and p-aminophenols. 3. The water-soluble metabolites affording aniline on acid hydrolysis were examined, and gamma-glutamylanilide (23% of dose) was identified as a tick metabolite (40 mug dose). This metabolite was not found in extracts of dosed spiders.