ten Hagen T L, van Vianen W, Heremans H, Bakker-Woudenberg I A
Dept. Clinical Microbiology and Antimicrobial Therapy, Erasmus University Rotterdam, The Netherlands.
Liver. 1998 Oct;18(5):299-305. doi: 10.1111/j.1600-0676.1998.tb00809.x.
AIMS/BACKGROUND: We have previously shown a striking heterogeneity of naive murine Kupffer cells (KC) that depends on cell size.
In the present study, we demonstrate a shift in response of KC fractions separated on cell size by countercurrent elutriation upon priming with tumor necrosis factor-alpha (TNF-alpha) or interferon-gamma (IFN-gamma).
Whereas unprimed large KC are most active in the production of TNF-alpha and nitric oxide (NO), after priming of KC with TNF-alpha predominantly small and intermediate sized KC produce TNF-alpha in response to bacteria. Priming with IFN-gamma enhanced NO production in all KC. A strong synergy, with respect to production of NO, was observed when KC subfractions were exposed to a combination of TNF-alpha and IFN-gamma. Concerning TNF-alpha production, priming of KC subfractions seemed to induce a shift of activity from large KC to smaller KC.
The present data demonstrate a clear heterogeneity among murine KC with respect to immunologic response to stimuli. These results demonstrate that KC have different functions in immunologic reactions that seem to be related to size.
目的/背景：我们之前已表明，天然小鼠库普弗细胞（KC）存在显著的异质性，这取决于细胞大小。
在本研究中，我们证明了通过逆流淘析按细胞大小分离的KC组分在用肿瘤坏死因子-α（TNF-α）或干扰素-γ（IFN-γ）刺激后反应的转变。
未刺激的大型KC在TNF-α和一氧化氮（NO）产生方面最活跃，在用TNF-α刺激KC后，主要是小型和中型KC对细菌产生TNF-α。用IFN-γ刺激可增强所有KC中NO的产生。当KC亚组分暴露于TNF-α和IFN-γ的组合时，在NO产生方面观察到强烈的协同作用。关于TNF-α的产生，KC亚组分的刺激似乎诱导了活性从大型KC向小型KC的转变。
目前的数据表明，小鼠KC在对刺激的免疫反应方面存在明显的异质性。这些结果表明，KC在免疫反应中具有不同的功能，这似乎与细胞大小有关。
