Terada T, Kato M, Horie S, Endo K, Kitamura Y
Second Department of Pathology, Tottori University Faculty of Medicine, Yonago, Japan.
Liver. 1998 Oct;18(5):313-9. doi: 10.1111/j.1600-0676.1998.tb00811.x.
AIMS/BACKGROUND: This study was conducted to evaluate the expression of pancreatic digestive enzymes in hilar bile ducts and hepatocytes during human fetal liver organogenesis.
We investigated the expression of pancreatic alpha-amylase protein and messenger RNA (mRNA) in hilar primitive bile ducts and hepatocytes by immunohistochemistry and in situ hybridization techniques, using 11 human fetal livers of various gestational ages. The specificity of the immunohistochemistry and in situ hybridization procedures was confirmed by Western blot analysis and in situ hybridization using sense probes, respectively.
Immunoreactivity of pancreatic alpha-amylase protein and expression of pancreatic alpha-amylase mRNA were present not only in the primitive ductal cells of the hilar region including the ductal plate, remodelling bile ducts and remodeled bile ducts but also in primitive hepatocytes of the hilar region, though the immunoreactivity and mRNA signals in the primitive hepatocytes disappeared in the third trimester. There was perfect correlation between immunohistochemistry and in situ hybridization.
These results suggest that primitive biliary cells and hepatocytes of the hilar region in the human fetus do express pancreatic alpha-amylase protein and mRNA, and that the primitive biliary epithelial cells and hepatocytes in the hilar region share a common cell lineage with exocrine pancreatic cells.
目的/背景：本研究旨在评估人胎儿肝脏器官发生过程中肝门部胆管和肝细胞中胰腺消化酶的表达情况。
我们运用免疫组织化学和原位杂交技术，对11例不同孕周的人胎儿肝脏肝门部原始胆管和肝细胞中胰腺α淀粉酶蛋白和信使核糖核酸（mRNA）的表达进行了研究。免疫组织化学和原位杂交程序的特异性分别通过蛋白质免疫印迹分析和使用正义探针的原位杂交得以证实。
胰腺α淀粉酶蛋白的免疫反应性和胰腺α淀粉酶mRNA的表达不仅存在于肝门区包括胆管板、重塑胆管和已重塑胆管的原始导管细胞中，也存在于肝门区的原始肝细胞中，不过原始肝细胞中的免疫反应性和mRNA信号在妊娠晚期消失。免疫组织化学和原位杂交之间存在完美的相关性。
这些结果表明，人胎儿肝门区的原始胆管细胞和肝细胞确实表达胰腺α淀粉酶蛋白和mRNA，并且肝门区的原始胆管上皮细胞和肝细胞与胰腺外分泌细胞具有共同的细胞谱系。
