Wilkemeyer M F, Charness M E
Neurology Service, West Roxbury VA Medical Center, Massachusetts 02132, USA.
J Neurochem. 1998 Dec;71(6):2382-91. doi: 10.1046/j.1471-4159.1998.71062382.x.
Ethanol inhibits L1-mediated cell-cell adhesion in fibroblast cell lines stably transfected with human L1. Here we show that this action of ethanol is present in only a subset of transfected NIH/3T3 and L cell clonal cell lines. All L1-expressing cell lines had higher levels of cell adhesion than cell lines transfected with empty vector. In all ethanol-sensitive cell lines, L1-mediated adhesion was inhibited by ethanol (IC50 5-10 mM), 2 mM butanol, but not 5 mM pentanol. In contrast, ethanol-insensitive cell lines were not inhibited by up to 200 mM ethanol, 2 mM butanol, or 5 mM pentanol. Ethanol sensitivity or insensitivity was a stable property of each cell line and was not associated with differences in electrophoretic mobility, abundance, or cell surface localization of L1. Fab fragments prepared from anti-L1 polyclonal antisera inhibited cell adhesion only in the ethanol-sensitive cell lines. These data suggest that L1 may exist in an alcohol-sensitive or an alcohol-insensitive state that may be governed by host cell factors.
乙醇可抑制在稳定转染了人L1的成纤维细胞系中L1介导的细胞间黏附。在此我们表明，乙醇的这种作用仅存在于一部分转染的NIH/3T3和L细胞克隆细胞系中。所有表达L1的细胞系的细胞黏附水平均高于用空载体转染的细胞系。在所有对乙醇敏感的细胞系中，L1介导的黏附受到乙醇（IC50为5 - 10 mM）、2 mM丁醇的抑制，但不受5 mM戊醇的抑制。相比之下，对乙醇不敏感的细胞系即使在高达200 mM乙醇、2 mM丁醇或5 mM戊醇的情况下也不受抑制。乙醇敏感性或不敏感性是每个细胞系的稳定特性，并且与L1的电泳迁移率、丰度或细胞表面定位的差异无关。从抗L1多克隆抗体制备的Fab片段仅在对乙醇敏感的细胞系中抑制细胞黏附。这些数据表明，L1可能以对酒精敏感或不敏感的状态存在，这可能受宿主细胞因子的调控。
