Tobin D J, Alhaidari Z, Olivry T
Department of Biomedical Sciences, University of Bradford, England.
Exp Dermatol. 1998 Oct;7(5):289-97. doi: 10.1111/j.1600-0625.1998.tb00299.x-i1.
Several cases of an alopecia areata (AA)-like disease have been reported in mammalian species. How similar this disorder(s) is to human AA is unclear. We have previously shown that human AA is associated with antibodies to hair follicle (HF)-specific antigens and that similar antibody reactivities also occur in the C3H/HeJ "AA" murine model and in dogs with spontaneously occurring AA. The current preliminary study was conducted to determine whether a horse with AA-like hair loss contained circulating antibodies to HE The pathogenic potential of these antibodies was examined by passive transfer into anagen skin of C57BL/ 10 black mice. Indirect immunofluorescence analysis indicated that the equine "AA" serum reacted intensely with the inner root sheath, outer root sheath and pre-cortex of equine HF Immunoblot examination revealed antibodies to a 200-220 kDa doublet and to antigens of 40-60 kDa. Notably, this serum, but not control serum, contained antibodies that selectively immunoprecipitated trichohyalin from HF protein extracts. IgG fractions of serum obtained from an "AA" horse and from a normal control horse were injected into anagen murine skin. Histologically, normal hair regrowth was observed in mice injected with normal equine IgG. By contrast, hair did not re-grow in an area around the injection site of AA-treated mice even 13 weeks after first injection. This skin contained telogen follicles, most often without associated shafts, despite the presence of anagen HF in the remaining dorsum skin. While this study is preliminary, it demonstrates for the first time that antibodies to HF antigens are a feature of AA-like hair loss in horses. Some reactivities (e.g. against trichohyalin) were similar to those previously observed in "AA" dogs. Further, we provide in this pilot study preliminary evidence that such antibodies may disrupt hair re-growth when passively transferred into mice, supporting the view that anti-HF antibodies in AA may have pathogenic potential.
在哺乳动物物种中已报告了几例斑秃（AA）样疾病。这种疾病与人类斑秃的相似程度尚不清楚。我们之前已经表明，人类斑秃与毛囊（HF）特异性抗原的抗体有关，并且在C3H/HeJ“AA”小鼠模型和自发发生斑秃的犬类中也出现了类似的抗体反应。当前的初步研究旨在确定一匹患有AA样脱发的马是否含有针对HF的循环抗体。通过将这些抗体被动转移到C57BL/10黑色小鼠的生长期皮肤中来检测这些抗体的致病潜力。间接免疫荧光分析表明，马“AA”血清与马HF的内根鞘、外根鞘和皮质前层强烈反应。免疫印迹检查显示存在针对200 - 220 kDa双峰和40 - 60 kDa抗原的抗体。值得注意的是，这种血清而非对照血清含有能够从HF蛋白提取物中选择性免疫沉淀毛透明蛋白的抗体。从一匹“AA”马和一匹正常对照马获得的血清IgG组分被注射到生长期小鼠皮肤中。组织学上，注射正常马IgG的小鼠观察到正常的毛发生长。相比之下，即使在首次注射后13周，接受AA处理小鼠注射部位周围的区域毛发仍未重新生长。尽管其余背部皮肤存在生长期HF，但该皮肤含有休止期毛囊，且大多数情况下没有相关的毛干。虽然这项研究是初步的，但它首次证明了针对HF抗原的抗体是马AA样脱发的一个特征。一些反应性（例如针对毛透明蛋白的反应性）与之前在“AA”犬中观察到的相似。此外，我们在这项初步研究中提供了初步证据，即这种抗体在被动转移到小鼠体内时可能会干扰毛发再生，支持了AA中抗HF抗体可能具有致病潜力的观点。
