Martinez L G, Campíns-Falcó P, Sevillano-Cabeza A, Bosch-Reig F
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot/Valencia, Spain.
J Chromatogr B Biomed Sci Appl. 1998 Oct 23;718(1):143-51. doi: 10.1016/s0378-4347(98)00335-1.
Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.
头孢噻肟与1,2-萘醌-4-磺酸盐（NQS）进行衍生化反应，萃取至固相柱（C18）中，并用紫外-可见检测系统进行检测。该新方法的最佳条件为：碳酸氢盐-碳酸盐缓冲液，pH 10.5，25℃下反应5分钟，NQS浓度为7.1×10⁻³ mol l⁻¹。测试了液固法的准确性和精密度。该方法用于测定药物和尿液样本中的头孢噻肟。将所得结果与报道的尿液样本高效液相色谱法的结果进行对比。采用广义H点标准加入法测定尿液样本中的头孢噻肟。
