Jesionek-Kupnicka D, Buczyński J, Kordek R, Sobów T, Kłoszewska I, Papierz W, Liberski P P
Laboratory of Tumor Biology, School of Medicine, Lódź.
Folia Neuropathol. 1997;35(4):233-5.
The aim of our study was the estimation of the apoptosis process using in situ-end labelling of DNA breaks method on paraffin sections in 5 human cases of Alzheimer's disease (AD), 6 of Creutzfeldt-Jakob disease (CJD) and in 25 mice infected experimentally with the Fujisaki strain of CJD, killed sequentially at one-week intervals. The numbers of apoptotic cells in CJD-infected mice in the later stages of the disease and in terminally ill mice were progressively higher that at the early stage of the disease. Further, we found a correlation between the intensity of apoptosis and major lesions hallmark of disease--the intensity of spongiform changes in the cerebral cortex but not in the accumulation of PrP in CJD infected mice. The number of A beta-amyloid plaques in AD was not related to apoptotic index. Our study showed that apoptosis is a very important event in these neurodegenerative diseases and may become a basic mechanism in loss of neurons.
我们研究的目的是采用DNA断裂原位标记法，对5例人类阿尔茨海默病（AD）、6例克雅氏病（CJD）石蜡切片以及25只经实验感染CJD富士崎株且每隔一周依次处死的小鼠，评估细胞凋亡过程。在疾病后期的CJD感染小鼠和濒死小鼠中，凋亡细胞数量比疾病早期逐渐增多。此外，我们发现细胞凋亡强度与疾病主要病变特征——大脑皮质海绵状变化的强度相关，但与CJD感染小鼠中PrP的积累无关。AD中β-淀粉样斑块的数量与凋亡指数无关。我们的研究表明，细胞凋亡在这些神经退行性疾病中是一个非常重要的事件，可能成为神经元丧失的基本机制。
