Gale B K, Caldwell K D, Frazier A B
Department of Bioengineering, University of Utah, Salt Lake City 84112, USA.
IEEE Trans Biomed Eng. 1998 Dec;45(12):1459-69. doi: 10.1109/10.730439.
In this work, micromachining technologies are employed to develop a miniaturized electrical field-flow fractionation (EFFF) separation system. EFFF systems are used to separate colloidal particles such as cells, liposomes, proteins, or other particulates, and to characterize emulsions and other mixtures according to particle charge density. Macromachining techniques have been used to develop existing EFFF technologies. At the present time, the limiting factor in the development of higher precision EFFF separation systems has been the manufacturing approach. In this paper, the theory behind the operation and resolution of a micron-sized EFFF (mu-EFFF) system is described and the advantages to be gained from application of micromachining technologies are given, thus motivating the need for further miniaturization. A completely fabricated mu-EFFF system is developed, separations are performed, and the mu-EFFF system is compared to the theoretically predicted results as well as the results from current macro EFFF systems.
在这项工作中，采用微加工技术开发了一种小型化的电场流分级（EFFF）分离系统。EFFF系统用于分离细胞、脂质体、蛋白质或其他颗粒等胶体颗粒，并根据颗粒电荷密度对乳液和其他混合物进行表征。宏观加工技术已被用于开发现有的EFFF技术。目前，高精度EFFF分离系统发展的限制因素一直是制造方法。本文描述了微米级EFFF（μ-EFFF）系统的操作和分辨率背后的理论，并给出了应用微加工技术所获得的优势，从而激发了进一步小型化的需求。开发了一个完全制造好的μ-EFFF系统，进行了分离，并将μ-EFFF系统与理论预测结果以及当前宏观EFFF系统的结果进行了比较。
