他莫昔芬长期治疗诱导的大鼠附睾液成分变化。

Changes in the content of rat epididymal fluid induced by prolonged treatment with tamoxifen.

作者信息

Belmonte S, Maturano M, Bertini M F, Pusiol E, Sartor T, Sosa M A

机构信息

Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.

出版信息

Andrologia. 1998 Nov;30(6):345-50. doi: 10.1111/j.1439-0272.1998.tb01181.x.

DOI:10.1111/j.1439-0272.1998.tb01181.x

PMID:9835949

Abstract

Prolonged treatment with tamoxifen induces changes in the male reproductive tract in rats. In this study changes in the protein content of the rat epididymal fluid as a consequence of prolonged treatment with tamoxifen are reported. Among five lysosomal enzymes measured in the epididymal fluid, alpha-mannosidase (alpha-MAN) significantly diminished, but other enzymes did not. Electrophoretic analysis of fluids showed that proteins of estimated molecular weight 25, 60, 80-85 and 180 kDa decreased in the treated rats. We also detected an increase in the binding of beta-galactosidase (beta-GAL) to caudal spermatozoa in treated rats. These changes may be related in part to the loss of fertilizing capacity of spermatozoa after tamoxifen treatment.

摘要

他莫昔芬的长期治疗会诱导大鼠雄性生殖道发生变化。本研究报告了他莫昔芬长期治疗导致的大鼠附睾液蛋白质含量变化。在附睾液中检测的五种溶酶体酶中，α-甘露糖苷酶（α-MAN）显著减少，但其他酶没有变化。对附睾液的电泳分析表明，在接受治疗的大鼠中，估计分子量为25、60、80 - 85和180 kDa的蛋白质减少。我们还检测到在接受治疗的大鼠中，β-半乳糖苷酶（β-GAL）与尾部精子的结合增加。这些变化可能部分与他莫昔芬治疗后精子受精能力的丧失有关。