己巴比妥和苯胺与不同年龄的对照及苯巴比妥处理大鼠肝脏微粒体细胞色素P-450的结合。
The binding of hexobarbital and aniline to cytochrome P-450 of liver microsomes from control and phenobarbital-treated rats of different ages.
作者信息
Müller D, Klinger W
出版信息
Acta Biol Med Ger. 1976;35(5):627-33.
The spectral changes due to the binding of hexobarbital and aniline to cytochrome P-450 of rat liver microsomes were investigated in 10-day- to 15-month-old rats. The Ks values for both substances and consequently the affinity for cytochrome P-450 do not change during ageing. Phenobarbital treatment does not alter the affinity of hexobarbital, but enhances the Ks value for aniline. The maximal spectral changes (delta A max) due to aniline are nearly equal in all age groups whereas delta A max due to hexobarbital is very small in young rats and increases considerably during ageing. The age-dependence of the hexobarbital-induced delta A max is similar to the development of drug-metabolizing reactions. delta A max due to hexobarbital and aniline is enhanced by phenobarbital treatment of the rats. The addition of aniline to microsomes enhances the Ks value and diminishes delta A max for hexobarbital.
在10日龄至15月龄的大鼠中，研究了己巴比妥和苯胺与大鼠肝脏微粒体细胞色素P - 450结合引起的光谱变化。两种物质的Ks值以及因此对细胞色素P - 450的亲和力在衰老过程中不会改变。苯巴比妥处理不会改变己巴比妥的亲和力，但会提高苯胺的Ks值。各年龄组中，苯胺引起的最大光谱变化（ΔAmax）几乎相等，而己巴比妥引起的ΔAmax在幼鼠中非常小，且在衰老过程中显著增加。己巴比妥诱导的ΔAmax的年龄依赖性与药物代谢反应的发展相似。对大鼠进行苯巴比妥处理可增强己巴比妥和苯胺引起的ΔAmax。向微粒体中添加苯胺会提高Ks值并降低己巴比妥的ΔAmax。
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