Goldman H B, Israeli R S, Lerner J L, Hollabaugh R S, Steiner M S
University of Tennessee Urologic Research Laboratories and Department of Urology, University of Tennessee, Memphis 38163, USA.
Urology. 1998 Dec;52(6):1073-8. doi: 10.1016/s0090-4295(98)00358-6.
To evaluate the effect of prostate biopsy on the prostate-specific antigen (PSA) reverse transcriptase-polymerase chain reaction (RT-PCR) test.
Ninety men who were scheduled to undergo prostate biopsy because of an elevated PSA or abnormal digital rectal examination, or both, were recruited to have PSA RT-PCR performed on peripheral blood samples drawn before and at 30 minutes, 1 week, and 1 month after undergoing prostate biopsy. PSA RT-PCR was performed and all PCR products were blotted and hybridized with phosphorus-32 (32-P)-PSA cDNA probe (exon 3 to 5).
Of 90 patients, 77 had a negative prebiopsy PSA RT-PCR result. Of these 77, 2 (2.6%) had a positive PSA RT-PCR result at some point after the biopsy procedure. Both of these patients had no evidence of malignancy on biopsy. The PSA RT-PCR test was positive at 30 minutes for 1 patient, but was negative at 1 week; the other was positive at 1 week but the patient did not return for the 1-month sample.
Our study indicates that 2.6% of patients with an initially negative PSA RT-PCR will have a positive PSA RT-PCR test after biopsy has been performed. Although this is uncommon, it may have profound implications for those patients in whom it occurs. On the basis of our results, it appears that one should wait longer than 1 week after prostate biopsy before obtaining blood for PSA RT-PCR testing to decrease the likelihood of a spurious PSA RT-PCR result.
评估前列腺活检对前列腺特异性抗原（PSA）逆转录聚合酶链反应（RT-PCR）检测的影响。
招募90名因PSA升高或直肠指检异常或两者皆有而计划接受前列腺活检的男性，在前列腺活检前、活检后30分钟、1周和1个月采集外周血样本进行PSA RT-PCR检测。进行PSA RT-PCR检测，所有PCR产物进行印迹，并与磷-32（32-P）-PSA cDNA探针（外显子3至5）杂交。
90例患者中，77例活检前PSA RT-PCR结果为阴性。在这77例患者中，2例（2.6%）在活检后某个时间点PSA RT-PCR结果为阳性。这2例患者活检均无恶性肿瘤证据。1例患者在30分钟时PSA RT-PCR检测为阳性，但1周时为阴性；另1例在1周时为阳性，但该患者未返回采集1个月时的样本。
我们的研究表明，初始PSA RT-PCR结果为阴性的患者中，2.6%在进行活检后PSA RT-PCR检测会呈阳性。虽然这种情况不常见，但对发生这种情况的患者可能有深远影响。根据我们的结果，似乎在前列腺活检后应等待超过1周再采集血液进行PSA RT-PCR检测，以降低出现假阳性PSA RT-PCR结果的可能性。
