de Vet E C, van den Bosch H
Centre for Biomembranes and Lipid Enzymology, Institute for Biomembranes, Utrecht University, Utrecht, The Netherlands.
Biochem Biophys Res Commun. 1998 Nov 27;252(3):629-33. doi: 10.1006/bbrc.1998.9670.
The nucleotide sequence is reported of alkyl-dihydroxyacetonephosphate synthase cDNA from the cellular slime mold Dictyostelium discoideum. The open reading frame encodes a protein of 611 amino acids which shows a 33% amino acid identity to the human enzyme. This D. discoideum homolog carries a variant of the peroxisomal targeting signal type 1 at its C-terminus (PKL). Expression of the cDNA in Escherichia coli yielded an enzymatically active protein.
报道了来自细胞黏菌盘基网柄菌的烷基二羟基丙酮磷酸合酶cDNA的核苷酸序列。开放阅读框编码一个由611个氨基酸组成的蛋白质，该蛋白质与人类酶的氨基酸同一性为33%。这种盘基网柄菌的同源物在其C末端携带1型过氧化物酶体靶向信号的变体（PKL）。该cDNA在大肠杆菌中的表达产生了一种具有酶活性的蛋白质。
