Wimmer K, Vinatzer U, Zwirn P, Fonatsch C, Wieser R
Institut für Medizinische Biologie der Universität Wien, Währingerstrasse 10, Wien, A-1090, Austria.
Biochem Biophys Res Commun. 1998 Nov 27;252(3):691-6. doi: 10.1006/bbrc.1998.9588.
An alternative form of the transcription factor EVI1, MDS1-EVI1, which previously had been believed to exist only in the context of leukemic fusion mRNAs, has recently been shown to be expressed also in normal human tissues. Moreover, it acts as an antagonist of EVI1, activating transcription of reporter constructs repressed by EVI1. We cloned the murine homolog of MDS1-EVI1 as well as mMds1 and show localization of mMds1 close to mEvi1 on chromosome 3. Using RT-PCR, we demonstrate widespread expression of both Evi1 forms in the adult mouse, as well as their upregulation during in vitro hematopoietic differentiation. Our data underscore the biological importance of both EVI1 and MDS1-EVI1 and provide the basis for further studies of their function in the mouse model system.
转录因子EVI1的一种替代形式，即MDS1-EVI1，此前一直被认为仅在白血病融合mRNA的背景下存在，最近研究表明它也在正常人体组织中表达。此外，它作为EVI1的拮抗剂，可激活被EVI1抑制的报告基因构建体的转录。我们克隆了MDS1-EVI1的小鼠同源物以及mMds1，并显示mMds1在3号染色体上与mEvi1位置相近。通过逆转录聚合酶链反应（RT-PCR），我们证明了Evi1的两种形式在成年小鼠中广泛表达，并且在体外造血分化过程中上调。我们的数据强调了EVI1和MDS1-EVI1的生物学重要性，并为在小鼠模型系统中进一步研究它们的功能提供了基础。
