Qian Y C, Fan C Y, Gong Y, Yang S L
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, PR China.
Biochim Biophys Acta. 1998 Nov 26;1443(1-2):233-8. doi: 10.1016/s0167-4781(98)00218-8.
The novel cDNAs encoding four long neurotoxin homologues were firstly cloned from the venom gland of Naja naja atra by reverse transcriptase-polymerase chain reaction. Amino acid sequence comparison showed that they may come from two different evolutionary origins. The mature proteins were expressed as soluble fusion proteins in Escherichia coli and purified for immunoblotting. The results revealed that they displayed different immunochemical properties.
通过逆转录聚合酶链反应首次从眼镜蛇毒腺中克隆出编码四种长神经毒素同源物的新cDNA。氨基酸序列比较表明它们可能来自两个不同的进化起源。成熟蛋白在大肠杆菌中表达为可溶性融合蛋白，并进行纯化用于免疫印迹。结果显示它们具有不同的免疫化学性质。
