小鼠Slug（Slugh）基因的基因组结构、表达及染色体定位

Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene.

作者信息

Jiang R, Norton C R, Copeland N G, Gilbert D J, Jenkins N A, Gridley T

机构信息

The Jackson Laboratory, 600 Main St., Bar Harbor, MN 04609, USA.

出版信息

Biochim Biophys Acta. 1998 Nov 26;1443(1-2):251-4. doi: 10.1016/s0167-4781(98)00225-5.

DOI:10.1016/s0167-4781(98)00225-5

PMID:9838149

Abstract

The Slug gene encodes a zinc finger protein implicated in the generation and migration of neural crest cells in several vertebrate species. Here we describe the genomic organization and chromosomal localization of the mouse Slug (Slugh) gene. The mouse Slug gene consists of three exons spanning approx. 4 kb. Northern blot analysis of RNA isolated from several tissues of adult mice revealed the presence of a single 2.1 kb transcript. The chromosomal location of mouse Slug was determined by interspecific backcross analysis. The mapping results indicated that Slugh is located in the proximal region of mouse chromosome 16.

摘要

Slug基因编码一种锌指蛋白，在几种脊椎动物物种中，该蛋白与神经嵴细胞的产生和迁移有关。在此，我们描述了小鼠Slug（Slugh）基因的基因组结构和染色体定位。小鼠Slug基因由三个外显子组成，跨度约为4 kb。对成年小鼠多个组织分离的RNA进行Northern印迹分析，结果显示存在一条单一的2.1 kb转录本。通过种间回交分析确定了小鼠Slug的染色体定位。定位结果表明，Slugh位于小鼠16号染色体的近端区域。