Palta P, Bansal N, Prakash B S, Manik R S, Madan M L
Embryo Biotechnology Centre, National Dairy Research Institute, Karnal, India.
Indian J Exp Biol. 1998 Aug;36(8):768-74.
The objective of this study was to measure the concentrations of immunoreactive inhibin (ir-inhibin) in follicular fluid from individual ovarian follicles and relate them to follicular diameter and follicular fluid concentrations of estradiol-17 beta, progesterone and testosterone in buffalo. Follicular size was measured with an ultrasound machine and follicles were categorized as small (4 to 5 mm diam.), medium (6 to 9 mm diam.) and large (10 mm and above in diam.). Ir-inhibin concentrations varied markedly between and within follicles of same size category. Follicular fluid ir-inhibin concentrations (microgram/ml) were positively related to follicular diameter (R = 0.32, n = 262, P < 0.001) and were significantly higher (P < 0.05) in large (8.32 +/- 0.56) in comparison to medium (7.02 +/- 0.31) follicles which, in turn had inhibin concentrations significantly higher (P < 0.001) than those in small follicles (5.13 +/- 0.48). Ir-inhibin and estradiol-17 beta concentrations were positively related in medium (R = 0.38, n = 128, P < 0.001) and large (R = 0.64, n = 35, P < 0.001) but not in small follicles. There was a negative relationship between ir-inhibin and progesterone concentrations in large follicles (R = 0.46, n = 33, P < 0.01), with no relationship between the two hormones in small and medium follicles. Ir-inhibin was positively related to molar ratios of estradiol-17 beta to progesterone in medium (R = 0.30, n = 124, P < 0.01) and large (R = 0.49, n = 24, P < 0.01) but not in small follicles. There was no relationship between ir-inhibin and testosterone concentrations in follicles of all size categories. The results of the present study suggest that follicular inhibin production is related to follicular size as well as intrafollicular estradiol-17 beta and progesterone concentrations.
本研究的目的是测定水牛单个卵巢卵泡卵泡液中免疫反应性抑制素（ir-抑制素）的浓度，并将其与卵泡直径以及卵泡液中雌二醇-17β、孕酮和睾酮的浓度相关联。用超声仪测量卵泡大小，并将卵泡分为小卵泡（直径4至5毫米）、中卵泡（直径6至9毫米）和大卵泡（直径10毫米及以上）。同一大小类别的卵泡之间以及卵泡内部，ir-抑制素浓度差异显著。卵泡液ir-抑制素浓度（微克/毫升）与卵泡直径呈正相关（R = 0.32，n = 262，P < 0.001），与中卵泡（7.02 +/- 0.31）相比，大卵泡（8.32 +/- 0.56）中的ir-抑制素浓度显著更高（P < 0.05），而中卵泡的抑制素浓度又比小卵泡（5.13 +/- 0.48）显著更高（P < 0.001）。中卵泡（R = 0.38，n = 128，P < 0.001）和大卵泡（R = 0.64，n = 35，P < 0.001）中，ir-抑制素与雌二醇-17β浓度呈正相关，但小卵泡中无此关系。大卵泡中ir-抑制素与孕酮浓度呈负相关（R = 0.46，n = 33，P < 0.01），小卵泡和中卵泡中这两种激素之间无相关性。中卵泡（R = 0.30，n = 124，P < 0.01）和大卵泡（R = 0.49，n = 24，P < 0.01）中，ir-抑制素与雌二醇- /孕酮的摩尔比呈正相关，但小卵泡中无此关系。所有大小类别的卵泡中，ir-抑制素与睾酮浓度均无相关性。本研究结果表明，卵泡抑制素的产生与卵泡大小以及卵泡内雌二醇-17β和孕酮浓度有关。
