Pinzani P, Petruzzi E, Orlando C, Gallai R, Serio M, Pazzagli M
Department of Clinical Pathophysiology, University of Florence, Italy.
J Biolumin Chemilumin. 1998 Sep-Oct;13(5):321-5. doi: 10.1002/(SICI)1099-1271(1998090)13:5<321::AID-BIO495>3.0.CO;2-5.
Free radicals are considered to be important factors involved in many physiopathological processes. Several methods have been proposed for studying the mechanisms of antioxidant protection against free radical-induced injury, including the measurement of the total antioxidant capacity (TAC) in body fluids, based on enhanced chemiluminescence. This technique is calibrated against Trolox and assay results are expressed as mumol/L of Trolox equivalents. Since many of the complications induced by diabetes appear to be mediated by oxygen free radical generation, we have investigated serum antioxidant capacity in a group of healthy subjects and in insulin-dependent diabetic (IDDM) subjects. A statistically significant difference was noticed in TAC values between the IDDM group and the young control group. Even if the biological meaning of this significant reduction in TAC remains to be explained, an overproduction of precursors of reactive oxygen free radicals and/or a decreased scavenger systems efficiency can be associated with the increased risk of atherosclerotic cardiovascular disease in diabetic patients.
自由基被认为是参与许多生理病理过程的重要因素。已经提出了几种方法来研究抗氧化剂对自由基诱导损伤的保护机制，包括基于增强化学发光法测量体液中的总抗氧化能力（TAC）。该技术以Trolox为标准进行校准，测定结果以μmol/L的Trolox当量表示。由于糖尿病引发的许多并发症似乎是由氧自由基生成介导的，我们研究了一组健康受试者和胰岛素依赖型糖尿病（IDDM）受试者的血清抗氧化能力。IDDM组和年轻对照组之间的TAC值存在统计学上的显著差异。即使TAC显著降低的生物学意义仍有待解释，但活性氧自由基前体的过度产生和/或清除系统效率的降低可能与糖尿病患者动脉粥样硬化性心血管疾病风险增加有关。
