Morishita M, Yamamura T, Bachchu M A, Shimazu A, Iwamoto Y
Department of Preventive Dentistry, Hiroshima University School of Dentistry, Japan.
Arch Oral Biol. 1998 Apr;43(4):329-33. doi: 10.1016/s0003-9969(97)00114-3.
The purpose was to investigate the effects of oestradiol on the function of periodontal ligament (PDL) cells by measuring the production of osteocalcin in vitro. Cells were obtained from the healthy periodontal ligament of teeth extracted from two males and two females for orthodontic reasons. Serum-free medium was used when testing the effects of oestradiol on PDL cells. The amount of osteocalcin in the culture medium was analysed by two-step sandwich enzyme immunoassay in the presence or absence of oestradiol. It was shown that oestradiol enhanced the production of osteocalcin by PDL cells in a time- and dose-dependent manner. PDL cells obtained from both male and female donors were affected by oestradiol. It thus appears that oestradiol is one of the factors important for PDL cells to express their function.
目的是通过体外测量骨钙素的产生来研究雌二醇对牙周膜（PDL）细胞功能的影响。细胞取自因正畸原因拔除的两名男性和两名女性牙齿的健康牙周膜。在测试雌二醇对PDL细胞的影响时使用无血清培养基。在有或没有雌二醇存在的情况下，通过两步夹心酶免疫测定法分析培养基中骨钙素的含量。结果表明，雌二醇以时间和剂量依赖性方式增强了PDL细胞骨钙素的产生。来自男性和女性供体的PDL细胞均受雌二醇影响。因此，雌二醇似乎是PDL细胞发挥其功能的重要因素之一。
