Endo Y, Mizuno T, Nishimura Y, Goto Y, Watari T, Tsujimoto H, Hasegawa A
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Vet Immunol Immunopathol. 1998 Oct 23;65(2-4):113-23. doi: 10.1016/s0165-2427(98)00147-0.
cDNA clones of feline chemokines, MIP-1alpha, MIP-1beta and RANTES, were molecularly isolated with the purpose of using these sequences for future investigation of the inhibitory effects on lentivirus entry and their role in immunological functions. The feline MIP-1alpha and MIP-1beta cDNA clones spanned their entire coding regions encoding 93 and 92 amino acids, respectively. The amino acid sequences of feline MIP-1alpha and MIP-1beta compared to those of their human, mouse and rat counterparts showed similarities of 75.3-79.6% and 73.9-88.0%, respectively. Feline MIP-1alpha and MIP-1beta had four conserved cysteines with a structure made up of the first two cysteines that are characteristic of the CC-chemokine subfamily. The amino terminal of these MIP-1alpha and MIP-1beta sequences was distinctly hydrophobic, suggesting that they may function as signal peptides. A partial cDNA clone consisting of 193 bp was obtained for feline RANTES, and it also showed a high degree of sequence similarity to those of other species and contained the characteristic structure made up of adjacent cysteines. These molecular clones of feline chemokines will be useful in the examination of their inhibitory effect on the cellular entry of feline immunodeficiency virus.
猫趋化因子MIP-1α、MIP-1β和RANTES的cDNA克隆被分子分离出来，目的是利用这些序列，以便未来研究它们对慢病毒进入的抑制作用及其在免疫功能中的作用。猫MIP-1α和MIP-1β的cDNA克隆跨越了它们各自的整个编码区，分别编码93和92个氨基酸。猫MIP-1α和MIP-1β的氨基酸序列与人类、小鼠和大鼠对应物的氨基酸序列相比，相似度分别为75.3%-79.6%和73.9%-88.0%。猫MIP-1α和MIP-1β有四个保守的半胱氨酸，其结构由前两个半胱氨酸组成，这是CC趋化因子亚家族的特征。这些MIP-1α和MIP-1β序列的氨基末端明显具有疏水性，表明它们可能作为信号肽发挥作用。获得了一个由193 bp组成的猫RANTES部分cDNA克隆，它与其他物种的序列也显示出高度的相似性，并包含由相邻半胱氨酸组成的特征结构。这些猫趋化因子的分子克隆将有助于研究它们对猫免疫缺陷病毒细胞进入的抑制作用。
