Brosious E M, Morrison B Y, Schmidt R M
Am J Clin Pathol. 1976 Nov;66(5):878-82. doi: 10.1093/ajcp/66.5.878.
Although the isopropanol precipitation test is a commonly used and sensitive test for detecting unstable hemoglobins, false-positive reactions are frequently observed. In this study, the storage temperature of the test samples, type of stored sample amount of fetal hemoglobin (Hb F) in the sample, and presence of potassium cyanide (KCN) in the hemolysate preparation all affected the accuracy of the test. The addition of 2% KCN to samples before testing eliminated or greatly reduced false-positive reactions. Hb F levels greater than 4% caused false-positive results. When specimens with Hb F levels less than 4% were stored at 4C and as whole blood, they showed no false-positive reaction for as long as two weeks. However, duplicate specimens stored as whole blood but unrefrigerated and those stored as hemolysates showed false-positive reactions after three days.
尽管异丙醇沉淀试验是检测不稳定血红蛋白常用且灵敏的试验，但经常观察到假阳性反应。在本研究中，试验样品的储存温度、储存样品的类型、样品中胎儿血红蛋白（Hb F）的含量以及溶血产物制备中氰化钾（KCN）的存在均影响试验的准确性。在检测前向样品中添加2%的KCN可消除或大大减少假阳性反应。Hb F水平大于4%会导致假阳性结果。当Hb F水平低于4%的标本作为全血储存在4℃时，长达两周都未出现假阳性反应。然而，作为全血储存但未冷藏的重复标本以及作为溶血产物储存的标本在三天后出现了假阳性反应。
