Sawada Y, Kuroda Y, Sashio H, Yamamoto N, Tonokatsu Y, Sakagami T, Fukuda Y, Shimoyama T, Nishigami T, Uematsu K
Fourth Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
J Gastroenterol. 1998;33 Suppl 10:22-5.
We have established a Helicobacter pylori-infected Mongolian gerbil model, following Hirayama's method, to clarify gastric diseases associated with H. pylori infection. We administered the culture broth of H. pylori ATCC 43504 orally to 8-week-old male Mongolian gerbils. After H. pylori inoculation, the gerbils were fed in a vinyl isolator. Subsequently, over the course of 48 weeks, they were killed for histopathological examination, H. pylori culture, and serum antibody measurement. H. pylori colonization in the glandular stomach was seen in all the infected gerbils, but only a few H. pylori were detected histologically. The serum antibody titer in the H. pylori-inoculated group increased gradually in comparison with controls. Acute inflammation, immature epithelium, and erosion were observed 2 weeks after H. pylori infection. Chronic inflammation was noted from 4 weeks after H. pylori infection. We also found intestinal metaplasia and gastric ulcers from 12 and 24 weeks after inoculation, respectively. Some histological findings were similar to those in humans, but the chronic inflammation in the gerbils was present mainly in the deep mucosa and submucosa. This appears to be a good animal model for H. pylori-associated gastric diseases and it may be useful for investigating the pathogenesis of H. pylori infection.
我们按照平山的方法建立了幽门螺杆菌感染的蒙古沙鼠模型，以阐明与幽门螺杆菌感染相关的胃部疾病。我们将幽门螺杆菌ATCC 43504的培养液口服给8周龄的雄性蒙古沙鼠。接种幽门螺杆菌后，沙鼠在乙烯隔离器中饲养。随后，在48周的时间里，将它们处死以进行组织病理学检查、幽门螺杆菌培养和血清抗体检测。在所有感染的沙鼠中都观察到幽门螺杆菌在腺胃中的定植，但组织学上仅检测到少量幽门螺杆菌。与对照组相比，接种幽门螺杆菌组的血清抗体滴度逐渐升高。幽门螺杆菌感染2周后观察到急性炎症、不成熟上皮和糜烂。幽门螺杆菌感染4周后出现慢性炎症。我们还分别在接种后12周和24周发现了肠化生和胃溃疡。一些组织学发现与人类相似，但沙鼠中的慢性炎症主要存在于深层黏膜和黏膜下层。这似乎是一个研究幽门螺杆菌相关胃部疾病的良好动物模型，可能有助于研究幽门螺杆菌感染的发病机制。
