Chakroun C, Grimont F, Urdaci M C, Bernardet J F
Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Jouy-en-Josas Cedex, France.
Dis Aquat Organ. 1998 Jul 30;33(3):167-77. doi: 10.3354/dao033167.
Flavobacterium psychrophilum is the agent of cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. Ribosomal RNA gene restriction patterns (ribotypes) and plasmid profiles were determined on a collection of 85 strains isolated from different countries and fish species. Several ribotypes were obtained by using the restriction endonucleases Hinc II and Pvu II. Computer analysis of the ribotypes revealed that some of them were clearly associated with the fish species from which the strains were isolated, whereas no correlation with the geographical origin was found. Most of the strains harboured at least one plasmid and several different plasmid profiles were observed, even among strains sharing the same ribotype. These methods, used alone or in combination with other typing techniques, can be considered powerful tools for the epidemiological tracing of F. psychrophilum infections.
嗜冷黄杆菌是全球鲑科鱼类冷水病和虹鳟鱼苗综合征的病原体。对从不同国家和鱼类物种分离得到的85株菌株进行了核糖体RNA基因限制性图谱（核糖型）和质粒图谱分析。使用限制性内切酶Hinc II和Pvu II获得了几种核糖型。对核糖型的计算机分析表明，其中一些与分离菌株的鱼类物种明显相关，而未发现与地理来源有相关性。大多数菌株至少携带一个质粒，即使在具有相同核糖型的菌株中也观察到几种不同的质粒图谱。这些方法单独使用或与其他分型技术结合使用，可被视为嗜冷黄杆菌感染流行病学追踪的有力工具。
