Rozet J M, Gerber S, Perrault I, Calvas P, Souied E, Châtelin S, Molina-Gomez D, Munnich A, Kaplan J
Unité de Recherches sur les Handicaps Génétiques de l'Enfant, INSERM U-393, Paris Cedex 15, France.
Cytogenet Cell Genet. 1998;82(1-2):91-4. doi: 10.1159/000015072.
Glutathione is a ubiquitous antioxidant in mammalian tissues. The first step of its synthesis is catalyzed by the glutamate-cysteine ligase (GLCL) which consists of a heavy, catalytic subunit and a light, regulatory subunit (GLCLR). Previous genetic analyses have revealed frequent losses of chromosome 1p22-->p21 in human malignant mesothelioma and the shortest region of overlapping deletions has been narrowed between the two loci D1S435 and D1S236. An expressed sequence tag of the GLCLR gene was found within a YAC contig encompassing the same interval aoffwas therefore considered as a good candidate gene for predisposition to human mesothelioma. We report here the characterization of the genomic structure of the GLCLR gene and the refine its physical mapping to chromosome 1p22.1.
谷胱甘肽是哺乳动物组织中普遍存在的一种抗氧化剂。其合成的第一步由谷氨酸-半胱氨酸连接酶（GLCL）催化，该酶由一个重的催化亚基和一个轻的调节亚基（GLCLR）组成。先前的基因分析显示，人类恶性间皮瘤中常出现1p22→p21染色体缺失，两个位点D1S435和D1S236之间的最短重叠缺失区域已被缩小。在一个包含相同区间的酵母人工染色体（YAC）重叠群中发现了GLCLR基因的一个表达序列标签，因此被认为是人类间皮瘤易感性的一个良好候选基因。我们在此报告GLCLR基因的基因组结构特征，并将其物理定位精确到1p22.1染色体。
