Hochberg C J, Witheiler A P, Cook H
Am J Obstet Gynecol. 1976 Nov 1;126(5):531-4. doi: 10.1016/0002-9378(76)90743-2.
The quantitation of bilirubin in amniotic fluid is of paramount importance in prognosticating the severity of Rh isoimmunization. Amniotic fluid contaminated by maternal blood can result in erroneous results when direct spectrophotemetric analysis is performed on such fluid. The technique and results of a one-step chloroform extraction performed on "bloody" amniotic fluid is presented. Results confirm that chloroform extraction yields more accurate clinical information in regard to Rh isoimmunized patients in whom a "bloody tap" is obtained.
羊水胆红素定量对于预测Rh血型免疫的严重程度至关重要。当对被母体血液污染的羊水进行直接分光光度分析时，可能会得出错误结果。本文介绍了对“血性”羊水进行一步氯仿萃取的技术和结果。结果证实，对于通过“血性穿刺”获得羊水的Rh血型免疫患者，氯仿萃取能产生更准确的临床信息。
