Kim JC, Lund DB
Cornell University, College of Agriculture and Life Sciences, Ithaca, New York 14853, USA.
Biotechnol Prog. 1998 Nov;14(6):951-8. doi: 10.1021/bp9800992.
Adsorption kinetics of beta-lactoglobulin onto stainless steel at low concentrations were studied to determine adsorption rate constants for monolayer coverage. Experimental data obtained from a batch reactor were described quantitatively using a reversible adsorption model which becomes the Langmuir-type adsorption isotherm at equilibrium. The forward rate constant, k1, and backward rate constant, k2, were 10(5) mL g-1 min-1 and 5 x 10(-)3 min-1, respectively. A high-performance liquid chromatographic system using a stainless steel column packed with stainless steel particles (column adsorption reactor) was also used to measure adsorption with a step change of beta-lactoglobulin solution. The difference in behavior of an adsorption and a desorption isotherm in this column adsorption reactor suggested surface denaturation of beta-lactoglobulin after adsorption which was consistent with other research work. beta-lactoglobulin adsorption in the column adsorption reactor was affected by volumetric flow rate and concentration up to 4 mL min-1 and 100 &mgr;g mL-1, respectively.
研究了低浓度下β-乳球蛋白在不锈钢上的吸附动力学，以确定单层覆盖的吸附速率常数。使用可逆吸附模型对从间歇式反应器获得的实验数据进行定量描述，该模型在平衡时成为朗缪尔型吸附等温线。正向速率常数k1和反向速率常数k2分别为10(5) mL g-1 min-1和5×10(-)3 min-1。还使用了一个装有不锈钢颗粒的不锈钢柱的高效液相色谱系统（柱吸附反应器），通过β-乳球蛋白溶液的阶跃变化来测量吸附。该柱吸附反应器中吸附和解吸等温线行为的差异表明，吸附后β-乳球蛋白发生了表面变性，这与其他研究工作一致。柱吸附反应器中β-乳球蛋白的吸附分别受体积流速和浓度的影响，最高可达4 mL min-1和100 μg mL-1。
