嗜热链球菌及其他乳酸菌中质粒携带的应激蛋白基因分布

Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria.

作者信息

Somkuti G A, Steinberg D H

机构信息

U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.

出版信息

Curr Microbiol. 1999 Jan;38(1):43-7. doi: 10.1007/pl00006770.

DOI:10.1007/pl00006770

PMID:9841781

Abstract

The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from ca. 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. Southern blots with a rep probe showed that all hsp16.4+ plasmids in S. thermophilus strains also shared homology with the replication function (rep) of pER341, suggesting the common origin of these plasmids.

摘要

通过Southern杂交分析，在来自链球菌属（47株）、乳杆菌属（34株）、乳球菌属（24株）和明串珠菌属（5株）的总DNA提取物中检测热应激蛋白基因（hsp）的存在。由pER341（2.8 kb）的ORF2片段制备的生物素化hsp16.4探针与7株嗜热链球菌菌株和1株乳酸乳球菌亚种乳脂亚种的限制性DNA提取物呈阳性反应。在所有阳性嗜热链球菌菌株中，hsp位于大小约为2.8 kb至11 kb的质粒上，而hsp存在于乳酸乳球菌亚种乳脂亚种的一个7.5 kb质粒中。用rep探针进行的Southern印迹显示，嗜热链球菌菌株中的所有hsp16.4 +质粒也与pER341的复制功能（rep）具有同源性，表明这些质粒具有共同起源。