Woodbury R G, Wendin C, Clendenning J, Melendez J, Elkind J, Bartholomew D, Brown S, Furlong C E
Department of Medicine, University of Washington, Seattle 98195, USA.
Biosens Bioelectron. 1998 Nov 1;13(10):1117-26. doi: 10.1016/s0956-5663(98)00060-8.
Surface plasmon resonance (SPR) biosensors were constructed on miniature integrated sensors. Recognition elements were attached to the sensor surface using a gold-binding repeating polypeptide. Biosensors with fluorescyl groups attached to their surfaces were functional for at least 1 month of daily use with little decrease in response to the binding of an anti-fluorescyl monoclonal antibody. The coupling of protein A to the gold-binding polypeptide on the sensor surface enabled the biosensor to detect the binding of antibodies to the protein A and provided a sensor with convertible specificity. The system described herein provides a simple and rapid approach for the fabrication of highly specific, durable, portable and low cost SPR-based biosensors.
表面等离子体共振（SPR）生物传感器构建于微型集成传感器之上。识别元件通过一种与金结合的重复多肽附着于传感器表面。表面附着有荧光基团的生物传感器在每日使用的情况下可正常工作至少1个月，对抗荧光基团单克隆抗体结合的响应几乎没有下降。将蛋白A偶联到传感器表面的金结合多肽上，使得生物传感器能够检测抗体与蛋白A的结合，并提供了一种具有可转换特异性的传感器。本文所述系统为制造高特异性、耐用、便携且低成本的基于SPR的生物传感器提供了一种简单快速的方法。
