Wagner N, Löhler J, Tedder T F, Rajewsky K, Müller W, Steeber D A
Institute for Genetics, University of Cologne, Germany.
Eur J Immunol. 1998 Nov;28(11):3832-9. doi: 10.1002/(SICI)1521-4141(199811)28:11<3832::AID-IMMU3832>3.0.CO;2-J.
Mesenteric lymph nodes (MLN) drain the gut where nutritive antigens and pathogens are encountered by lymphocytes of the gut-associated lymphoid tissue. We sought to determine how lymphocytes enter the MLN by studying mice double deficient for beta7 integrins and L-selectin. beta7/L-selectin double-deficient lymphocytes did not migrate into MLN. Most importantly, MLN formation was drastically impaired in beta7/L-selectin double-deficient mice. Lymphocyte numbers in MLN from beta7/L-selectin double-deficient mice were tenfold reduced compared to control mice. A high percentage of the few lymphocytes still detected in MLN from beta7/L-selectin double-deficient mice were CD44hi CD18hi, suggesting alternate migration pathways independent of L-selectin and beta7 integrin for these cells. We conclude that the combination of both molecules, L-selectin and beta7 integrin, is indispensable for MLN formation and that these molecules may mediate lymphocyte migration to MLN in a sequential and synergistical manner.
肠系膜淋巴结（MLN）引流肠道，肠道相关淋巴组织的淋巴细胞在此接触营养性抗原和病原体。我们通过研究β7整合素和L-选择素双缺陷小鼠，试图确定淋巴细胞如何进入MLN。β7/L-选择素双缺陷淋巴细胞不会迁移到MLN中。最重要的是，β7/L-选择素双缺陷小鼠的MLN形成严重受损。与对照小鼠相比，β7/L-选择素双缺陷小鼠MLN中的淋巴细胞数量减少了十倍。在β7/L-选择素双缺陷小鼠的MLN中仍检测到的少数淋巴细胞中，有很大比例是CD44hi CD18hi，这表明这些细胞存在独立于L-选择素和β7整合素的替代迁移途径。我们得出结论，L-选择素和β7整合素这两种分子的组合对于MLN形成是不可或缺的，并且这些分子可能以顺序和协同的方式介导淋巴细胞向MLN的迁移。
